Dihydroethidium(Hydroethidine; PD-MY 003)是过氧化物指示剂，在细胞质中具有蓝色荧光，直到被氧化 (Ex/Em=518/616 nm)。
| 生物活性 | Dihydroethidium(Hydroethidine; PD-MY 003) is a superoxide indicator; exhibits blue-fluorescence in the cytosol until oxidized, where it intercalates within the cell's DNA, staining its nucleus a bright fluorescent red (Ex/Em=518/616 nm). |
| 分子量 | |
| 性状 | |
| Formula | |
| CAS 号 | |
| Emission (Em) | Em 380-450 nm Violet; Em 590-620 nm Orange |
| Excitation (Ex) | Ex<380 nm Ultraviolet; Ex 380-450 nm Violet; Ex 495-570 nm Green |
| 中文名称 | |
| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | 4°C, protect from light *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light) |
| 溶解性数据 | In Vitro: DMSO : ≥ 50 mg/mL(158.52 mM) H2O : 0.67 mg/mL(2.12 mM;Need ultrasonic) *"≥" means soluble, but saturation unknown. 配制储备液 | 1 mM | 3.1705 mL | 15.8524 mL | 31.7048 mL | | 5 mM | 0.6341 mL | 3.1705 mL | 6.3410 mL | | 10 mM | 0.3170 mL | 1.5852 mL | 3.1705 mL |
*请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (protect from light)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。 In Vivo: 请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂： ——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶 1. 请依序添加每种溶剂： 10% DMSO 40%PEG300 5%Tween-80 45% saline Solubility: ≥ 2.5 mg/mL (7.93 mM); Clear solution
此方案可获得 ≥ 2.5 mg/mL (7.93 mM，饱和度未知) 的澄清溶液。 以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。 *以上所有助溶剂都可在本网站选购。
|
| 染色示例 | Description: Dihydroethidium is a superoxide indicator with red fluorescence, it can be used to detect ROS production.Method: For the detection of ROS production in liver tissues.1. Frozen sections of liver tissues in each group (5-μm-thick) are prepared and incubated with Dihydroethidium (7.5 mM; 30 min; 37℃; dark).2. The sections are observed using a fluorescence microscope (Leica, Germany) and the percentage of Dihydroethidiumpositive cells is calculated through a quantitative morphometric analysis. Description: Dihydroethidium is a superoxide indicator, it can be used to detect superoxide anion production. Dihydroethidium specifically reacts with intracellular superoxide anion and turns into ethidium in nuclei with red fluorescence. Description: Dihydroethidium is a superoxide indicator with red fluorescence, it can be used for the estimation of reactive oxygen species (ROS) levels.Method: For paraffin sections staining.1. Paraffin sections (5 μM) are used to measure retinal and choroidal ROS production with Dihydroethidium (5 μmol/L; 20 min; 37℃).2. To confirm the specificity of Dihydroethidium staining, eye slides are preincubated with 100 U/mL polyethylene glycol-conjugated superoxide dismutase (30 min; 37℃).3. Use an Olympus DP73 fluorescence microscope (Tokyo, Japan) and Image J-NIH freeware to measure the intensity of staining. Description: Dihydroethidium is a superoxide indicator with red fluorescence, it can be used to indicate the ROS accumulation.Method: For brain sections staining.1. Incubate brain sections in 10 μM Dihydroethidium solution (1 h; 37℃; dark).2. Wash sections with PBS for 3 times.3. Samples are mounted with neutral balata and observed with the fluorescent microscope (Carl Zeiss, Weimar, Germany)and the positive area is calculated using Image J.
|
m.cnreagent.com
