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Vitamin D3
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Vitamin D3图片
CAS NO:67-97-0
包装与价格:
包装价格(元)
100mg电议
1 g电议
5 g电议
10 g电议
50 g电议

产品名称
维生素D3
Cholecalciferol
Colecalciferol
产品介绍
Vitamin D3 (Cholecalciferol; Colecalciferol) 是维生素 D 的天然存在形式,代谢激活后能诱导细胞分化和癌细胞增殖。
生物活性

Vitamin D3 (Cholecalciferol; Colecalciferol) is a naturally occuring form of vitamin D. Vitamin D3 induces cell differentiation and prevents proliferation ofcancercells.

IC50& Target

Human Endogenous Metabolite

 

体外研究
(In Vitro)

Vitamin D3 is an inactive vitamin D molecule in vivo. Vitamin D3 undergoes two hydroxylation processes to activate it. Vitamin D3 is first hydroxylated in the liver to form the circulating prohormone 25-hydroxy vitamin D3 [25(OH)D3] by the enzyme 25-hydroxylase (CYP27A1) and probably also by other enzymes (e.g., CYP2R1)[1].
The second hydroxylation occurs in the kidneys via the enzyme 1-alpha-hydroxylase, yielding 1,25- dihydroxycholecalciferol (calcitriol), which is the biologically active form of vitamin D[1].
Vitamin D3 (2-10 μM; 24-48 hours) exhibits anti-proliferative effects in a dose- and time- dependent manner. Maximal reduction of viability post treatment of 62% (IK), 52% (RL-95-2), and 55% (Hec-1A) occurrs by 72 h of treatment with 10 μM Vitamin D3. but 24-hour exposure lacks significant reduction in viable cells[2].
Cholecalciferol (10 μM; 24-48 hours) shows marked increases in nuclear VDR staining and produces local VDR activation in IK cells[1].

Cell Viability Assay[2]

Cell Line:EC cell lines from EEC, Ishikawa 3-H-12(IK), RL-95/2, and HEC-1-A cells
Concentration:2-10 μM
Incubation Time:24-72 hours
Result:Reduced viability in response to VD3 in a dose- and time-dependent manner.
Indicated that the conversion of VD3 to 25(OH)D is an essential step for the reduced cell viability effect.

Cell Viability Assay[2]

Cell Line:EC cell lines from EEC, Ishikawa 3-H-12(IK) cells
Concentration:10 μM
Incubation Time:24-48 hours
Result:Improved nuclear VDR content in IK cells.
体内研究
(In Vivo)

Cholecalciferol (oral gavage; 5 mg/kg; 7 days) potentiates the CCl4 toxicity only in the liver, as indicated by plasma levels of ALT and AST, biochemical markers of hepatic damage. It significantly increases renal calcium levels in mice, but renal calcium content does not differ significantly between mice[3].

Animal Model:Male ddY mice on CCl4 toxicity[3]
Dosage:5 mg/kg
Administration:Oral gavage; 5 mg/kg; 7 days
Result:Potentiated CCl4-induced hepatotoxicity and enhanced mouse mortality, without increasing renal toxicity and generation of liver fibrosis.
Clinical Trial
分子量

384.64

性状

Solid

Formula

C27H44O

CAS 号

67-97-0

中文名称

维生素D3;胆骨化醇

结构分类
  • Others
运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, protect from light, stored under nitrogen

*该产品在溶液状态不稳定,建议您现用现配,即刻使用。

溶解性数据
In Vitro: 

DMSO : 100 mg/mL(259.98 mM;Need ultrasonic)

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM2.5998 mL12.9992 mL25.9983 mL
5 mM0.5200 mL2.5998 mL5.1997 mL
10 mM0.2600 mL1.2999 mL2.5998 mL
*

请根据产品在不同溶剂中的溶解度,选择合适的溶剂配制储备液;该产品在溶液状态不稳定,建议您现用现配,即刻使用

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: 10% DMSO    40%PEG300   5%Tween-80   45% saline

    Solubility: ≥ 2.08 mg/mL (5.41 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (5.41 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH2O 中,得到澄清透明的生理盐水溶液
  • 2.

    请依序添加每种溶剂: 10% DMSO    90% (20%SBE-β-CDin saline)

    Solubility: 2.08 mg/mL (5.41 mM); Suspended solution; Need ultrasonic

    此方案可获得 2.08 mg/mL (5.41 mM) 的均匀悬浊液,悬浊液可用于口服和腹腔注射。

    以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
  • 3.

    请依序添加每种溶剂: 10% DMSO    90%corn oil

    Solubility: ≥ 2.08 mg/mL (5.41 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (5.41 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

*以上所有助溶剂都可在本网站选购。