5-Carboxyfluorescein diacetate N-succinimidyl ester is a cell permeable dye (Ex=492 nm, Em=517 nm). 5-Carboxyfluorescein diacetate N-succinimidyl ester can label cells by covalently binding to intracellular molecules. 5-Carboxyfluorescein diacetate N-succinimidyl ester is used to track lymphocyte migration and proliferation^[1].

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)^[1].
1. Dilute the stock solution in PBS (to give a 50 μM solution).
2. Add 110 μL of this solution per milliliter of cells (to give a final concentration of5 μM) and mix rapidly.
3. After5 minat room temperature, add 10 vol of PBS containing 5% FBS.
4. Centrifuge cells 5 min at 300 ×g, 20 ℃.
5. Remove the supernatant, and wash threetimes, each time by resuspending in 10 vol PBS containing 5% FBS.
6. Centrifuge cells 5 min at 300 × g, 20 ℃, and remove the supernatant.
Note: Labeling occurs rapidly, and it is essential that the tracker is dispersed as evenly and quickly as possible so that cells are uniformly labeled.

557.46

Solid

C₂₉H₁₉NO₁₁

150206-05-6

5-羧基二乙酸荧光素 N-琥珀酰亚胺酯

Room temperature in continental US; may vary elsewhere.

DMSO : 20.83 mg/mL(37.37 mM;ultrasonic and warming and heat to 60℃)

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。