Pembrolizumab

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Pembrolizumab

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:

1374853-91-4

包装与价格：

包装	价格(元)
1mg	电议
5mg	电议
25mg	电议
50mg	电议
100mg	电议
200mg	电议

产品名称

MK-3475
Lambrolizumab

产品介绍

Pembrolizumab (MK-3475) 是一种人源化 IgG4 抗体，为PD-1的抑制剂，用于癌症免疫的研究。

生物活性

Pembrolizumab (MK-3475) is a humanizedIgG4antibody inhibiting the programmed cell death 1 (PD-1) receptor, used incancer immunotherapy.

IC₅₀& Target

IC50: 0.6 nM (PD-1)

体外研究
(In Vitro)

Pembrolizumab (MK-3475) increases the secretion of cytokines IFN-γ, TNF-α and the apoptotic cell death of A549 cells^[2].
Pembrolizumab improves the αROR1-CAR T-mediated cytotoxicity, and reduces tumorigenesis in the co-cultured αROR1-CAR T and A549 cells by blocking PD-1::PD-L1 interaction in αROR1-CAR T cells^[2].

Cell Cytotoxicity Assay^[2]

Cell Line:	αROR1-CAR T and A549 cells
Concentration:	10 μg/mL
Incubation Time:	Adding Pembrolizumab after A549-Red-Fluc cells preconditioned in elevated pressure (+ 100 mmHg) for 7 days.
Result:	Enhanced αROR1-CAR T killing of A549 lung cancer cells under elevated pressure.

Apoptosis Analysis^[2]

Cell Line:	A549 cells
Concentration:	10 μg/mL
Incubation Time:
Result:	Increased the apoptotic cell death in A549 cells, and improve the αROR1-CAR T-mediated cytotoxicity.

体内研究
(In Vivo)

Pembrolizumab inhibits tumor size in A549 xenograft mice pretreated by pressure^[2].

Animal Model:	Six-week-old male BALB/c athymic nude mice^[2]
Dosage:	5 mg/kg
Administration:	Pembrolizumab (5 mg/kg; Intravenous injection; Once)
Result:	Reduced tumorigenesis in αROR1-CAR T cells and co-cultured with A549 cells.

Clinical Trial

分子量

146266.23

性状

Liquid

CAS 号

1374853-91-4

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.