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Destruxin B
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Destruxin B图片
CAS NO:2503-26-6
包装与价格:
包装价格(元)
1mg电议
5mg电议
10mg电议
50mg电议

产品名称
黑僵菌素 B
产品介绍
Destruxin B 是从昆虫病原真菌中分离的绿僵菌 (Metarhizium anisopliae),是一种具有杀虫和抗癌活性的环肽。 Destruxin B 通过Bcl-2 家族依赖的线粒体途径在人类非小细胞肺癌细胞中诱导细胞凋亡。Destruxin B 显著激活caspase-3,并在体外和体内通过 caspase 介导的细胞凋亡减少肿瘤细胞增殖。
生物活性

Destruxin B, isolated from entomopathogenic fungusMetarhizium anisopliae, is one of the cyclodepsipeptides with insecticidal and anticancer activities. Destruxin B inducesapoptosisvia aBcl-2 Family-dependent mitochondrial pathway in human nonsmall cell lungcancercells[1]. Destruxin B significantly activatescaspase-3and reduces tumor cell proliferation through caspase-mediatedapoptosis, not only in vitro but also in vivo[2].

IC50& Target

Bcl-2

 

Caspase-3

 

体外研究
(In Vitro)

Destruxin B (1-30 μM; 48 hours) inhibits cell proliferation of H1299 cells and A549 cells, with IC50s of 4.1 μM and 4.9 μM, respectively[1].
Activation of mitochondria-dependent caspase cascade plays an important role in Destruxin B (1-30 μM; 48 hours) induced apoptotic cell death in A549 cells[1].
Destruxin B (1.25-20.00 μM; 72 hours) treatment significantly inhibits HT-29 human colorectal cancer cells viability in time- and dose-dependent manners[2].
Destruxin B (10 or 20 μM; 12 and 24 hours) treatment markedly increases levels of the proapoptotic protein PUMA and reduces levels of antiapoptotic proteins Mcl-1 of A549 cells in a concentration- and time-dependent manner[1].

Cell Proliferation Assay[1]

Cell Line:H1299 cells
Concentration:1, 5, 10, 20, 30 μM
Incubation Time:48 hours
Result:Inhibited H1299 cells proliferation of H1299 cells with an IC50of 4.1 μM.

Apoptosis Analysis[1]

Cell Line:Lung adenocarcinoma A549 cells
Concentration:1, 5, 10, 20, 30 μM
Incubation Time:48 hours
Result:Induces caspase-dependent Lung adenocarcinoma A549 cells death.

Cell Viability Assay[2]

Cell Line:HT-29 human colorectal cancer cells
Concentration:1.25, 2.50, 5.00, 10.00 and 20.00 μM
Incubation Time:72 hours
Result:The IC50s at 24 h, 48 h and 72h were determined as 14.97, 2.00 and 0.67 μM, respectively.

Western Blot Analysis[1]

Cell Line:A549 cells
Concentration:10 or 20 μM
Incubation Time:12 and 24 hours
Result:Regulates Mcl-1 and PUMA.
体内研究
(In Vivo)

Destruxin B (DB) ( injection; 0.6-15 mg/kg/day for 6 weeks) attenuates the tumor growth in time- and dose-dependent manners[2].

Animal Model:Athymic female nude mice (BALB/cAnN.Cg-Foxn1nu/CrlNarl), approximately 4-5 weeks old on arrival[2]
Dosage:Low dose (0.6 mg/kg), medium dose (3 mg/kg), high dose (15 mg/kg)
Administration:Injection; daily; for 6 weeks
Result:Low dose, medium dose and high dose indicated a reduction of 23.9%, 33.4% and 55.8% in mean tumor size, respectively.
分子量

621.81

性状

Solid

Formula

C32H55N5O7

CAS 号

2503-26-6

中文名称

黑僵菌素 B

结构分类
  • Cyclopeptides
来源

Metarhizium anisopliae

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder-20°C3 years
4°C2 years
In solvent-80°C6 months
-20°C1 month