L-Glutamine (L-Glutamic acid 5-amide) is a non-essential amino acid present abundantly throughout the body and involved in many metabolic processes. L-Glutamine provides a source of carbons for oxidation in some cells^[1][2].

L-Glutamine is important as a precursor for peptide and protein synthesis, amino sugar synthesis, purine and pyrimidine and thus nucleic acid and nucleotide synthesis, and also provides a source of carbons for oxidation in some cells. L-Glutamine is the most abundant extracellular amino acid in vivo (0.7 mM compared to an approximate L-glutamate concentration of 20 μM)^[1].
In BRIN-BD11 cells, culture for 24 h with 10 mM L-Glutamine compared with 1 mM resulted in substantial changes in gene expression with 148 genes upregulated more than 1.8-fold, and 18 downregulated more than 1.8-fold, including many genes involved in cellular signaling, metabolism, gene regulation, and the insulin-secretory response. L-Glutamine increases the activity of the Ca2+ regulated phosphatase calcineurin and the transcription factor Pdx1^[2].

146.14

Solid

C₅H₁₀N₂O₃

56-85-9

L-谷氨酰胺

• Amino acids

Room temperature in continental US; may vary elsewhere.

H₂O : 33.33 mg/mL(228.07 mM;ultrasonic and adjust pH to 1 with HCl)

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

• 1.

  请依序添加每种溶剂： PBS

  Solubility: 7.69 mg/mL (52.62 mM); Clear solution; Need ultrasonic and warming and heat to 60℃