Sphingosine-1-phosphate (S1P) is an agonist ofS1P_1-5receptorsand a ligand of GPR3,GPR6and GPR12. Sphingosine-1-phosphate is an intracellular second messenger and mobilizes Ca²⁺as an extracellular ligand for G protein-coupled receptors^[1]. Sphingosine-1-phosphate is an important lipid mediator generated from Sphingomyelin (HY-113498) or other membranephospholipids^[2].

S1P (1 μM) induces a significant Ca²⁺releases in HEK293 cells under serum starvation conditions (1% FCS)^[1].
In a functional Ca²⁺assay, Suramin (HY-B0879) alone does not exert any effect on intracellular Ca²⁺release via gpr3, gpr6 or gpr12. In contrast, S1P (1 μM) induces Ca²⁺release of gpr3, gpr6 and gpr12 in the presence of Suramin (HY-B0879) various concentrations in transfected HEK293 cells^[2].
In a functional Ca²⁺assay,S1P (3-3000 nM) in the presence Suramin (300 μM), exhibits nanomolar EC₅₀values for gpr3 (EC₅₀=29 nM), gpr6 (EC₅₀=15 nM) and gpr12 (EC₅₀=24 nM), rat gpr3 (EC₅₀=68 nM),respectively in HEK293 cells^[2].
S1P increases intracellular calcium levels in TAg-Jurkat cells expressing S1P1 and G_qi5, which allows for phospholipase C stimulation by G_i proteins, when used at a concentration of 200 nM, as well as in TAg-Jurkat cells expressing S1P2 and S1P3 receptors (EC₅₀s = 8 and 11 nM, respectively)^[3].

379.47

Solid

C₁₈H₃₈NO₅P

26993-30-6

• Ketones, Aldehydes, Acids

Room temperature in continental US; may vary elsewhere.

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

DMSO : 5 mg/mL(13.18 mM;ultrasonic and warming and adjust pH to 4 with HCl and heat to 80℃)

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。