Uridine-5'-diphosphate disodium salt 是一种有效的选择性P2Y6受体激动剂 (EC50=300 nM; 对人 P2Y6受体 pEC50=6.52)。Uridine-5'-diphosphate disodium salt 是一种内源性代谢产物 (endogenous metabolite)，催化多种底物的葡糖醛酸糖化反应，可用于核酸生物合成 (RNA biosynthesis)。
| 生物活性 | Uridine-5'-diphosphate disodium salt is a potent, selectiveP2Y6receptor native agonist (EC50=300 nM;pEC50=6.52 for human P2Y6receptor). Uridine-5'-diphosphate disodium salt, anendogenous metabolite, catalyzes the glucuronidation of a wide array of substrates and is used innucleic acid (RNA) biosynthesis[1][2]. |
| IC50& Target[1] | Human Endogenous Metabolite |
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体外研究
(In Vitro) | Uridine-5'-diphosphate disodium salt (100 μM; for 15 min) significantly induces microglial CCL2 and CCL3 mRNA expression[2].
Uridine-5'-diphosphate disodium salt (100 μM; 3 hours) induces chemokine expression in microglia[2].
Uridine-5'-diphosphate disodium salt (100 μM; 0.5, 1, 3, 6 12 hours) induces expression of mRNA encoding CCL2 and CCL3 within 30 min, and such expression reaches maximal levels at 1 h, returning to basal levels at 3-12 h[2].
Uridine-5'-diphosphate disodium salt (10, 100, 1000 μM; 3 hours) induces a concentration-dependent increase in the expression of chemokines at both the mRNA and protein level[2].
Uridine-5'-diphosphate disodium salt (100 μM; for 15 min) induces activation of NFATc1 and NFATc2 in microglia[2].
Uridine-5'-diphosphate disodium salt is a competitive antagonist at the humanP2Y14receptor (pEC50=7.28) but not rat P2Y14receptor[1].
UDP-glucose (UDPG; HY-N7032) is a potent P2Y14agonist[3][4].
RT-PCR[2] | Cell Line: | Primary microglia | | Concentration: | 100 μM | | Incubation Time: | For 15 min | | Result: | Significantly induced microglial CCL2 and CCL3 mRNA expression. |
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| 结构分类 | - Ketones, Aldehydes, Acids
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | -20°C, sealed storage, away from moisture *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture) |
| 溶解性数据 | In Vitro: H2O : 100 mg/mL(223.15 mM;Need ultrasonic) DMSO : 1 mg/mL(2.23 mM;Need ultrasonic) 配制储备液 | 1 mM | 2.2315 mL | 11.1577 mL | 22.3155 mL | | 5 mM | 0.4463 mL | 2.2315 mL | 4.4631 mL | | 10 mM | 0.2232 mL | 1.1158 mL | 2.2315 mL |
*请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。 In Vivo: 请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂： ——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶 1. 请依序添加每种溶剂： PBS Solubility: 100 mg/mL (223.15 mM); Clear solution; Need ultrasonic
*以上所有助溶剂都可在本网站选购。 |
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