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EWP 815
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
EWP 815图片
CAS NO:20231-01-0
包装与价格:
包装价格(元)
25mg电议
50mg电议
100mg电议

产品介绍
EWP 815 是一种二硫类似物,是Ins(1,4)P2磷酸酶和Ins(1,4,5)P35-磷酸酶的有效抑制剂。EWP 815 也参与抑制体内多巴胺酶 β-羟化酶活性。
生物活性

EWP 815 is a disulfiram analogue, is a potent inhibitor ofIns(1,4)P2phosphataseandIns(1,4,5)P35-phosphatase. EWP 815 also inhibits enzymedopamine β-hydroxylaseactivity[1][2].

IC50& Target

Ins(1,4)P2 phosphatase; Ins(1,4,5)P3 5-phosphatase[1]; dopamine β-hydroxylase[2]

体外研究
(In Vitro)

EWP 815 inhibits thyrotropin-releasing hormone (TRH; 0.03 mM)-stimulated inositol phospholipid breakdown without affecting basal breakdown rates in prelabelled GH3cells with IC50s of 83 mM (soluble enzymes) and 71 mM (particulate enzymes), respectively[1].
EWP 815 (30 μM; 1 h) exerts higher inhibition on Ins(1,4)P2phosphatase rather than Ins(1,4,5)P35-phosphatase with the mean [3H]Ins(1,4)P2/mean [3H]InsPrecovery ratio of 2.6[1].
EWP 815 (6, 8 μM; 10 min) inhibits dephosphorylation of [3H]Ins(1,4,5)P35-phosphatase in particulate and soluble fractions with IC50s of 6 μM and 8 μM, respectively[1].
EWP 815 (3-300 μM; 30 min) suppresses thyrotropin-releasing hormone (TRH)-stimulated (100 nM) inositol phospholipid production by 30% (100 μM) and 1.8% (300 μM), respectively, without affecting Ins(1,4,5)P3binding[1].

体内研究
(In Vivo)

EWP 815 (50 mg/kg; i.p.; 30 min before killed) inhibits the enzyme dopamine β-hydroxylase activity in mice[2].

Animal Model:Female mice (20 g)[2]
Dosage:50 mg/kg
Administration:Intraperitoneal injection; 30 min before killed
Result:Intraperitoneal injection; 30 min before killedInhibited β-hydroxylase of3H-α-methyldopamine.
Reduced the amount of3H-α-Me-NA by 12%.
分子量

350.59

Formula

C12H22N4S4

CAS 号

20231-01-0

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.