包装 | 价格(元) |
10mM (in 1mL DMSO) | 电议 |
1g | 电议 |
5g | 电议 |
2-Deoxy-D-glucose (2DG), is a glucose analogue, act as competitive glycolytic inhibitor.
Cell lines | Cell lines SkBr3, MCF-7, BT474, and MDA/MB468, |
Preparation Method | Cells were plated at 4000 cells well-1 in a 96-well plate, allowed 24 h to attach and subsequently exposed for 4 days to 2-Deoxy-D-glucose. |
Reaction Conditions | 4-12 mM for 0-4 days |
Applications | Growth of MDA/MB468 cells was completely inhibited by treatment with 8 mM 2-Deoxy-D-glucose. Treatment with 4 mM 2-Deoxy-D-glucose resulted in approximately 30% inhibition of cell growth. Of the breast cancer cell lines tested, SkBr3 was the most sensitive to the growth inhibitory effects of 2-Deoxy-D-glucose. Cell growth was completely inhibited at 4 mM 2-Deoxy-D-glucose. |
Animal models | male Sprague-Dawley (SD) rats |
Preparation Method | Animals were handled routinely in order to reduce stress associated with injection. 2-deoxy-d-glucose (400 mg/kg), or saline, was administered intraperitoneally. |
Dosage form | Intraperitoneal injection , 400 mg/kg |
Applications | Ad concentrations increased from 2.73 ng/ml to 7.2 ng/ml while NAd concentrations increased from 1.8 ng/ml to 3.14 ng/ml after injected 2-deoxy-d-glucose. |
文献引用 | |
产品描述 | 2-Deoxy-D-glucose (2DG), is a glucose analogue, act as competitive glycolytic inhibitor[1]. 2-Deoxy-d-glucose (2DG) has been demonstrated to be a powerful agent for blocking and probing increased sugar metabolism in cancer cells[2]. Because of its similarity to glucose, 2-Deoxy-D-glucose inhibits glycolysis, but as chemically it is also 2-deoxymannose it is able to compete with mannose in the growing lipid-linked oligosaccharide chain during the initial steps of N-linked glycosylation. This mannose-like property of 2-Deoxy-D-glucose results in misfolded proteins leading to endoplasmic reticulum (ER) stress[3]. 2-Deoxy-D-glucose delivered in the diet produces cardiac toxicity in rats at doses ranging from 0.02 to 0.3 g/kg (0.04-0.6% 2-Deoxy-D-glucose by weight in the diet) and hastens mortality at doses above 0.2 g/kg (0.4% in the diet)[4]. 2-Deoxy-D-glucose evoked increases in plasma adrenaline and glucose at 20 and 60 min[5]. References: |