Arbutin (β-Arbutin) 是一种广泛存在的天然多酚,具有抗氧化,抗炎和抗肿瘤特性。是黑色素细胞中酪氨酸酶 (tyrosinase)的竞争性抑制剂,对单酚酶的Kiapp值为 1.42 mM;对二酚酶Kiapp的值为 0.9 mM。Arbutin 也用作脱色剂。
生物活性 | Arbutin (β-Arbutin) is a competitive inhibitor oftyrosinase, withKiappvalues of 1.42 mM for monophenolase; 0.9 mM for diphenolase. Arbutin is also used as depigmenting agents[1]. Arbutin is a natural polyphenol isolated from the bearberry plantArctostaphylos uvaursi, possesses with anti-oxidant, anti-inflammatory and anti-tumor properties[2][3]. |
IC50& Target | Human Endogenous Metabolite |
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体外研究 (In Vitro) | Arbutin (0.3-5.4 mM; 24 hours, 48 hours, 72 hours; B16 murine melanoma cells) inhibites the viability of B16 murine melanoma cells in a time-and dose-dependent manner[2]. Arbutin (1.4-5.4 mM; 24 hours) increases the apoptosis rate of B16 murine melanoma cell of treatment at a dose of 5.4 mM[2].
Cell Viability Assay[2] Cell Line: | B16 murine melanoma cells | Concentration: | 0.3 mM, 0.7 mM, 1.4 mM, 2.1 mM, 2.9 mM, 3.6 mM, 5.4 mM | Incubation Time: | 24 hours, 48 hours or 72 hours | Result: | Inhibited the viability of B16 murine melanoma cells in a time- and dose-dependent manner. |
Apoptosis Analysis[2] Cell Line: | B16 murine melanoma cells | Concentration: | 1.4 mM, 2.9 mM, 5.4 mM | Incubation Time: | 24 hours | Result: | Caused apoptosis in 19.3% of the cells. |
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体内研究 (In Vivo) | Arbutin (50 mg/kg, 100 mg/kg; oral administration; every day; for 17 days; male C57BL/6 mice) pretreatment exhibits markedly protective effects on ISO-induced cardiac hypertrophy in mice[3].
Animal Model: | Male C57BL/6 mice ( 20-25 g)[3] | Dosage: | 50 mg/kg, 100 mg/kg | Administration: | Oral administration; every day; for 17 days | Result: | Ameliorated the ISO-induced myocardial injury. |
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来源 | - Plants
- Saxifragaceae
- Bergenia purpurascens(Hook. f. et Thoms.) Engl.
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运输条件 | Room temperature in continental US; may vary elsewhere. |
储存方式 | Powder | -20°C | 3 years | | 4°C | 2 years | In solvent | -80°C | 6 months | | -20°C | 1 month |
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溶解性数据 | In Vitro: DMSO : ≥ 50 mg/mL(183.65 mM) H2O : 33.33 mg/mL(122.42 mM;Need ultrasonic) *"≥" means soluble, but saturation unknown. 配制储备液 1 mM | 3.6731 mL | 18.3655 mL | 36.7309 mL | 5 mM | 0.7346 mL | 3.6731 mL | 7.3462 mL | 10 mM | 0.3673 mL | 1.8365 mL | 3.6731 mL |
*请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。 In Vivo: 请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液,再依次添加助溶剂: ——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用;
以下溶剂前显示的百 分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶 1. 请依序添加每种溶剂: PBS Solubility: 100 mg/mL (367.31 mM); Clear solution; Need ultrasonic 2. 请依序添加每种溶剂: 10% DMSO 40%PEG300 5%Tween-80 45% saline Solubility: ≥ 2.5 mg/mL (9.18 mM); Clear solution
此方案可获得 ≥ 2.5 mg/mL (9.18 mM,饱和度未知) 的澄清溶液。 以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。 3. 请依序添加每种溶剂: 10% DMSO 90% (20%SBE-β-CDin saline) Solubility: ≥ 2.5 mg/mL (9.18 mM); Clear solution
此方案可获得 ≥ 2.5 mg/mL (9.18 mM,饱和度未知) 的澄清溶液。 以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。 4. 请依序添加每种溶剂: 10% DMSO 90%corn oil Solubility: ≥ 2.5 mg/mL (9.18 mM); Clear solution
此方案可获得 ≥ 2.5 mg/mL (9.18 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。 以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。 *以上所有助溶剂都可在本网站选购。
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