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Z-FA-FMK
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Z-FA-FMK图片
CAS NO:197855-65-5
规格:≥98%
包装与价格:
包装价格(元)
2mg电议
5mg电议
10mg电议
25mg电议
50mg电议
100mg电议

产品介绍
理化性质和储存条件
Molecular Weight (MW)386.42
FormulaC21H23N2O4F
CAS No.197855-65-5
Storage-20℃ for 3 years in powder form
-80℃ for 2 years in solvent
Solubility (In vitro)DMSO: 77 mg/mL (199.3 mM)
Water:<1 mg/mL
Ethanol: 34 mg/mL (88.0 mM)
Other info

Chemical Name: Z-FA-fluoromethyl ketone

InChi Key: ASXVEBPEZMSPHB-YJBOKZPZSA-N

InChi Code: InChI=1S/C21H23FN2O4/c1-15(19(25)13-22)23-20(26)18(12-16-8-4-2-5-9-16)24-21(27)28-14-17-10-6-3-7-11-17/h2-11,15,18H,12-14H2,1H3,(H,23,26)(H,24,27)/t15-,18-/m0/s1

SMILES Code: C[C@H](NC(=O)[C@H](Cc1ccccc1)NC(=O)OCc2ccccc2)C(=O)CF

Synonyms

Z-FA-fluoromethyl ketone; Z-FA-FMK;

实验参考方法
In Vitro

In vitro activity: Z-FA-FMK inhibits the degradation of fibrillar collagen by fibroblasts and osteoclasts. Z-FA-FMK inhibits LPS-induced cytokine production via inhibition of NF-kappaB-dependent gene expression in macrophages. Z-FA-FMK efficiently blocks T cell proliferation induced by mitogens and IL-2 in vitro.


Cell Assay: T cell proliferation following mitogen stimulation is determined using [3H]thymidine incorporation. In brief, PBMCs or purified T cells are seeded in a 96-well plate and stimulated with either PHA (5 μg/ml), costimulated with anti-CD3 mAb (5 μg/ml) and anti-CD28 mAb (2.5 μg/ml) or PMA plus ionomycin in the presence or absence of z-FA-FMK. The cells are cultured for 72 h with the last 16 h pulsed with [methyl-3H]thymidine (0.037 MBq). The cells are harvested onto glass fiber filter mats using a Tomtec automated multiwell harvester.

In VivoZ-FA-FMK significantly increases pneumococcal growth in both lungs and blood in a mouse model of intranasal pneumococcal infection. Z-FA-FMK blocks reovirus infection of Ras oncogenic tumours and host heart tissues in severe combined immunodeficiency mice.
Animal modelMice
Formulation & DosageN/A
References

Anal Biochem. 1985 Sep;149(2):461-5; J Immunol. 2006 Sep 15;177(6):3827-36.

生物活性

z-FA-FMK inhibits T cell proliferation and blasts formation at nontoxic concentrations. J Immunol. 2006 Sep 15;177(6):3827-36.

z-FA-FMK inhibits the production of IL-2 and IFN-γ in activated purified T cells. J Immunol.2006 Sep 15;177(6):3827-36.

The inhibition of T cell proliferation induced by z-FA-FMK is not reversed by exogenous rIL-2. J Immunol. 2006 Sep 15;177(6):3827-36.