Bromothymol Blue

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Bromothymol Blue

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:

76-59-5

包装与价格：

包装	价格(元)
10 mM * 1 mL in DMSO	电议
500mg	电议
1 g	电议
5 g	电议
10 g	电议

产品名称

溴百里酚蓝

产品介绍

Bromothymol Blue 是一种 pH 指示剂，是测定弱酸弱碱时使用的一种指示剂，其酸性时为黄色，碱性时为蓝色。

生物活性

Bromothymol Blue is a pH indicator.

体外研究
(In Vitro)

A first characterization and comparison is done by developing an easy and direct measurement method based on a pH indicator system using Bromothymol Blue (BTB) as the indicator and potassium phosphate as the buffer. A pH-shift assay is developed on the basis of BTB as the pH indicator and potassium phosphate as the buffer component^[1]. Three pH indicators are tested for the direct determination of 2- 2-keto-L-gulonic acid (2-KLG) production on a plate. The results show that Bromothymol Blue is superior to the other two indicators in terms of the obvious color change and a suitable pH range (blue to yellow at pH 6.5-7.5). Upon the addition of a Bromothymol Blue solution (0.1%, w/v) to an agar plate, zones surrounding colonies ofK. vulgare07 mutants change their color from blue to yellow becauseK. vulgare07 mutants release 2-KLG on agar plates, thereby acidifying surrounding areas around colonies^[2].

分子量

624.38

性状

Solid

Formula

C₂₇H₂₈Br₂O₅S

CAS 号

76-59-5

中文名称

溴麝香草酚蓝；溴百里酚蓝；溴百里香酚蓝；溴百里香酚蓝；溴化麝香草酚蓝；溴瑞香草酚蓝

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性数据

In Vitro:

DMSO : 100 mg/mL(160.16 mM;Need ultrasonic)

H₂O :< 0.1 mg/mL(insoluble)

配制储备液

浓度溶剂体积质量

1 mg

5 mg

10 mg

1 mM	1.6016 mL	8.0079 mL	16.0159 mL
5 mM	0.3203 mL	1.6016 mL	3.2032 mL
10 mM	0.1602 mL	0.8008 mL	1.6016 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (protect from light)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。