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Dihydrorhodamine 123
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Dihydrorhodamine 123图片
CAS NO:109244-58-8
包装与价格:
包装价格(元)
1mg电议
5mg电议
10mg电议

产品名称
二氢罗丹明123
DHR 123
产品介绍
Dihydrorhodamine 123 (DHR 123) 是一种荧光探针 (λex=488 nm,λem=525 nm)。
生物活性

Dihydrorhodamine 123 (DHR 123) is a fluorescent probe (λex=488 nm, λem=525 nm)[1].

体外研究
(In Vitro)

In the presence of 10 μM Dihydrorhodamine 123 (DHR 123) the stimulation of the neutrophil NADPH oxidase by the addition of 50 nM phorbol 12-myristate 13-acetat (PMA) resultes in an increase in the rate of rhodamine generation. The fluorescent intensity of the cells, in the presence of 10 μM Dihydrorhodamine 123, increases with time following the addition of 50 nM PMA. In the presence of 10 μM Dihydrorhodamine 123, induced HL60 cells show a sustained increase in fluorescence following the addition of 50 nM PMA[1].

分子量

346.38

性状

Solid

Formula

C21H18N2O3

CAS 号

109244-58-8

Emission (Em)
Em 495-570 nm Green
Excitation (Ex)
Ex 495-570 nm Green
运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20°C, protect from light

*该产品在溶液状态不稳定,建议您现用现配,即刻使用。

溶解性数据
In Vitro: 

DMSO : 100 mg/mL(288.70 mM;Need ultrasonic)

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM2.8870 mL14.4350 mL28.8700 mL
5 mM0.5774 mL2.8870 mL5.7740 mL
10 mM0.2887 mL1.4435 mL2.8870 mL
*

请根据产品在不同溶剂中的溶解度,选择合适的溶剂配制储备液;该产品在溶液状态不稳定,建议您现用现配,即刻使用

染色示例
  • Description: Dihydrorhodamine 123 can be used as a fluorescence probe for detecting ROS and ER stress with green fluorescence.
    Method: For cell labeling.
    1. Incubate cells with Dihydrorhodamine 123 (10 μM; 20 min).
    2. Use a confocal laser scanning microscopy for image (Olympus, Japan).
  • Description: Dihydrorhodamine 123 can be used as a fluorescence probe for detecting ion in cells with green fluorescence.
  • Description: Dihydrorhodamine 123 can be used as a fluorescence probe for detecting ROS with green fluorescence.
    Method: For cell labeling.
    1. Incubate cells with Dihydrorhodamine 123 (10 μM; 30 min).
    2. Use a laser confocal microscope for image (Delta Vision TM Elite, GE).