Indocyanine green (Foxgreen) is a low toxicic fluorescent agent that has been widely used in medical diagnostics, such as determining cardiac output, hepatic function, and liver blood flow, and for ophthalmic angiography.

Indocyanine green (Foxgreen)-photodynamic therapy (ICG-PDT) at concentrations 1000 μg/mL, induces the significant expression of BAX in HGF cells; however, the laser irradiation as well as ICG shows no significant effects on the expression of BCL-2 gene^[1].

774.96

Solid

C₄₃H₄₇N₂NaO₆S₂

3599-32-4

靛氰绿；吲哚菁绿

Room temperature in continental US; may vary elsewhere.

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

DMSO : 33.33 mg/mL(43.01 mM;Need ultrasonic)

H₂O : 5 mg/mL(6.45 mM;ultrasonic and warming and heat to 60℃)

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture and light)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

• 1.

  请依序添加每种溶剂： 10% DMSO    40%PEG300   5%Tween-80   45% saline

  Solubility: ≥ 2.5 mg/mL (3.23 mM); Clear solution

  此方案可获得 ≥ 2.5 mg/mL (3.23 mM，饱和度未知) 的澄清溶液。

  以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

  将 0.9 g 氯化钠，完全溶解于 100 mL ddH2O 中，得到澄清透明的生理盐水溶液
• 2.

  请依序添加每种溶剂： 10% DMSO    90% (20%SBE-β-CDin saline)

  Solubility: 2.5 mg/mL (3.23 mM); Suspended solution; Need ultrasonic

  此方案可获得 2.5 mg/mL (3.23 mM) 的均匀悬浊液，悬浊液可用于口服和腹腔注射。

  以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

  将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明

• 
  Description: Indocyanine green is a non-toxic biological dye, it only can be taken up by hepatocytes after intravenous injection, after which it is secreted by hepatocytes. When cultured with serum-free medium, hepatocytes take up Indocyanine green, and the cytoplasm appears green. After restoration of serum, Indocyanine green is secreted out of the cytoplasm.
  Method: For Indocyanine green uptake assay.
  1. Add Indocyanine green to serum-free hepatocyte medium with a final concentration of 1 mg/mL and cells are cultured with the medium for 1 hour at 37℃.
  2. Remove serum-free medium, and wash cells with PBS for three times.
  3. Cells are examined and imaged with a microscope (IX71FL, OLYMPUS).
• 
  Description: Indocyanine green is a non-toxic biological dye. Indocyanine green can be taken up by lymph and provides contrast for visualizing the lumen of lymphatic vessels using its single-photon fluorescence in both animal models and human subjects.
  Method: For noninvasive 2PF imaging.
• 
  Description: Indocyanine green is a non-toxic biological dye. Indocyanine green can be used to detect the distribution of polysaccharide-nanoemulsion following oral administration.
  Method: For polysaccharide-nanoemulsion distribution detecting.
  1. Indocyanine green is dissolved in the polysaccharide aqueous solution and then added to the nanoemulsion to form an complex (The final concentration of Indocyanine green in both solutions is 0.05 mg/mL).
  2. A total of 9 BALB/C nude mice are assigned to each of three experimental groups. The mice sre subjected to fasting 4 h following their respective treatment and then placed in a live imaging device (Lumina XR Series III; Perkin-Elmer, Inc., Waltham, MA, USA) to observe fluorescence distribution in vivo.