Gentamicin sulfate, an orally activeaminoglycosideantibiotic, inhibits the growth of both gram-positive and gram-negativebacteriaand to inhibit several strains of mycoplasma in tissue culture. Gentamicin sulfate inhibitsDNase Iwith anIC₅₀of 0.57 mM^[1][2][3][4].

Gentamicin is a more effectivein vitrobacterial inhibitor than combined penicillin-streptomycin, is nontoxic to tissue culture monolayers, and does not inhibit virus replication^[2].
Gentamicin has been used with success as an additive in commercial mycology media to inhibit growth of bacteria and has been shown to be bactericidal for a wider range of organisms (Pseudomonas aeruginosa,Proteus sp., andStreptococcus faecalis) than penicillin and streptomycin^[2].
Gentamicin does not interfere with the production of cytopathic effect by certain echoviruses and polioviruses in tissue culture, is nontoxic to Rhesus monkey kidney, HeLa, and human amnion cells, and is stable at autoclave temperatures^[2].
Gentamicin is produced by various species of the genusMicromonospora^[3].
Gentamicin C1a binds in the major groove of the A-site of the RNA^[3].

Gentamicin (oral and injectable forms) exhibits effective antibacterial activity againstYersinia pestisas demonstrated in a mouse infection model^[3].
Gentamicin (0.27 g/kg) shows a significant reduction of bacteria on the foreign body in mouse^[4].

561.65 (Average)

Solid

C₂₄H₅₅N₇O₁₁S^3*

1405-41-0

硫酸庆大霉素；庆大霉素硫酸盐；硫酸艮他霉素；硫酸正泰霉素；硫酸双生霉素；庆大霉素硫酸酯

• Antibiotics
• Other Antibiotics

Micromonospora purpurea

Room temperature in continental US; may vary elsewhere.

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

H₂O : ≥ 30 mg/mL

DMSO :< 1 mg/mL (ultrasonic;warming;heat to 60℃)(insoluble or slightly soluble)

*"≥" means soluble, but saturation unknown.

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

• 1.

  请依序添加每种溶剂： PBS

  Solubility: 100 mg/mL (Infinity mM); Clear solution; Need ultrasonic