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Spectinomycin
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Spectinomycin图片
CAS NO:1695-77-8
包装与价格:
包装价格(元)
100mg电议
250mg电议
500mg电议

产品介绍
Spectinomycin 是一种广谱抗生素,可以抑制多种革兰氏阳性和革兰氏阴性细菌的生长。Spectinomycin 通过特异靶向细菌核糖体,干扰蛋白质合成。Spectinomycin 也是 td 内含子 RNA 的非竞争性抑制剂。
生物活性

Spectinomycin is a broad-spectrumantibioticand inhibits the growth of a variety of gram-positive and gram-negative organisms. Spectinomycin acts by selectively targeting to thebacterialribosome and interrupting protein synthesis. Spectinomycin is also a noncompetitive inhibitor of td intron RNA[1][2][3][4][5].

IC50& Target

Aminoglycoside

 

体外研究
(In Vitro)

Spectinomycin selectively inhibits protein synthesis in cells and in extracts ofEscherichia coli.
Spectinomycin (50 μg/mL) inhibitsEscherichia coligrowth rapidly and reversibly, and suppresses amino acid incorporation immediately[1].
Spectinomycin (1 μg/mL or 3 μM) inhibits polypeptide synthesis directed either by endogenous messenger RNA or by MS-2 bacteriophage RNA, with maximum inhibition of 70-80% in extracts ofEscherichia coli[1].
Spectinomycin blocks the translocation of peptidyl-tRNAs from A-site to P-site by inhibiting the binding of elongation factor G to the ribosome[2].
Spectinomycin interacts specifically with the residues G1064 and 01192 in 16S rRNA and potentially makes it inactive[2].
Spectinomycin exhibits splicing inhibition and dependent on pH changes and Mg2+concentration, indicating electrostatic interactions with the intron RNA[3].

体内研究
(In Vivo)

Spectinomycin (20 mg/kg; i.m.; 20-100 mg/kg; 9 d) shows the safety in healthy chicks[4].
Spectinomycin (10 mg/kg; i.v.; single dose) has the major elimination pathway by renal excretion, approximately 55% is excreted into the urine in unchanged form[5].
Pharmacokinetics of Spectinomycin in Rat[5]

ParameterC0(μg/mL)AUC0-∞(μgoh/mL)Vd(L/kg)CL (L/h/kg)MRT (h)T1/2α(h)T1/2β(h)T1/2γ(h)feCLrenal(L/h/kg)Eratio
Non atrioventricular analysis44.316.80.7560.6020.757///0.5530.3591.00
Three-compartment model37.815.70.7470.6491.11/0.2370.75419.5//

Animal Model:Arbor Acres plus broiler chicks (15-day-old)[4]
Dosage:20 mg/kg, 60 mg/kg, 100 mg/kg
Administration:Intramuscular injection (chest muscles); 9 days
Result:Showed biosecurity of 20 mg/kg by complete blood count, biochemical parameters, histopathological, clinical signs, body weight gain, and feed conversion ratio (FCR).
Resulted minor toxicity of 60 mg/kg.
分子量

332.35

Formula

C14H24N2O7

CAS 号

1695-77-8

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.