Framycetin sulfate (Neomycin B sulfate) 是一种氨基糖苷类抗生素,是一种有效的RNase P裂解活性抑制剂,Ki为 35 μM。Framycetin sulfate 竞争 RNase P RNA 中特定的二价金属离子结合位点。Framycetin sulfate 抑制锤头状核酶 (hammerhead ribozyme),Ki值为 13.5 μM。Framycetin sulfate 是 5″-azido neomycin B 前体,与 miR-525 中的 Drosha 位点结合,可用于肝性脑病和肠致病性大肠杆菌感染。
生物活性 | Framycetin sulfate (Neomycin B sulfate), anaminoglycosideantibiotic, is a potentRNase P cleavage activityinhibitor with aKiof 35 μM. Framycetin sulfate competes for specific divalent metal ion binding sites in RNase P RNA. Framycetin sulfate inhibitshammerhead ribozymewith aKiof 13.5 μM. Framycetin sulfate, a 5″-azido neomycin B precursor, binds the Drosha site in miR-525 and is used for hepatic encephalopathy and enteropathogenicE. coliinfections[1][2]. |
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体外研究 (In Vitro) | The inhibition of RNase P RNA cleavage by Framycetin sulfate (Neomycin Bsulfate; Fradiomycin Bsulfate) is sensitive to pH and an increase in pH suppresses the inhibition in other systems[1]. Framycetin sulfate targets the bacterial and human ribosome and affect translation. 5″-azido neomycin B and Framycetin sulfate selectively inhibit production of the mature miRNA, boosts a downstream protein, and inhibits invasion in HCC cell line[2]. Framycetin sulfate binds to a structural rather than a sequence motif of the RNA. Its primary cognate target is the decoding site of the 16S rRNA, but it also binds to the Rev-responsive element in HIV-1, group I introns, and the hammerhead ribozyme, and thus inhibits their biological function[3]. Framycetin sulfate induces misreading of the genetic code during translation and inhibits several ribozymes. The ribosomal target site is the 16 S rRNA 1400 to 1500 region[4].
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运输条件 | Room temperature in continental US; may vary elsewhere. |
储存方式 | Powder | -20°C | 3 years | | 4°C | 2 years | In solvent | -80°C | 6 months | | -20°C | 1 month |
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溶解性数据 | In Vitro: H2O : 250 mg/mL(275.06 mM;Need ultrasonic) 配制储备液 1 mM | 1.1003 mL | 5.5013 mL | 11.0026 mL | 5 mM | 0.2201 mL | 1.1003 mL | 2.2005 mL | 10 mM | 0.1100 mL | 0.5501 mL | 1.1003 mL |
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以下溶剂前显示的百 分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶 1. 请依序添加每种溶剂: PBS Solubility: 50 mg/mL (55.01 mM); Clear solution; Need ultrasonic
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