Cefazolin (Cephazolin) 是第一代头孢菌素类抗生素，可用于多种细菌感染的研究。Cefazolin 具有抗炎作用，可以减轻术后认知功能障碍 (POCD)。
| 生物活性 | Cefazolin (Cephazolin) is a first-generationcephalosporinantibioticand can be used in varieties ofbacterialinfections research[1]. Cefazolin has anti-inflammatory effect and can attenuate post-operative cognitive dysfunction (POCD)[2]. |
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体外研究
(In Vitro) | Cefazolin (0-300 μg/ml; 6 or 24 h) has a direct anti-inflammatory effect on C8-B4 cells stimulated by lipopolysaccharide[2].
Cefazolin (0-400 μM; 72 h) treatment inhibits IL-2, IL-4 and IL-15-induced cell proliferation[3].
Cefazolin (100-400 μM; 30 min) treatment inhibits IL-2, IL-4, IL-15 and IL-21-stimulated JAK3 phosphorylation[3].
Cell Viability Assay[2] | Cell Line: | C8-B4 cells | | Concentration: | 0, 50, 100, 150, 200, 250, or 300 μg/ml | | Incubation Time: | 6 or 24 hours | | Result: | Inhibited the increase of IL-1β at all doses, but inhibited the increase of IL-6 only at 200 μg/ml. |
Cell Proliferation Assay[3] | Cell Line: | PBMC, and TF-1 cells | | Concentration: | 0, 100, 200, and 400 μM | | Incubation Time: | 72 hours | | Result: | Reduced IL-2, IL-4 and IL-15-induced cell proliferation, suggested that Cefazolin interferes not only with IL-15Rα, but also with IL-2/IL-15Rβ and/or γc. |
Cell Proliferation Assay[3] | Cell Line: | PBMC, NK-92, and TF-1 cells | | Concentration: | 0, 100, 200, and 400 μM | | Incubation Time: | 30 min | | Result: | Diminished the phosphorylation of JAK3 in response to the cytokine treatment, concluded suppressing signal transduction by γcreceptors. |
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体内研究
(In Vivo) | Cefazolin sodium pentahydrate (Subcutaneous injection; 300-500 mg/kg; once daily; 5 d) treatment improves learning and memory in mice after surgery[2].
| Animal Model: | 6- to 8-week-old male CD-1 mice underwent clinical exploratory laparotomy[2] | | Dosage: | 300-500 mg/kg | | Administration: | Subcutaneous injection; 300-500 mg/kg; once daily; 5 days | | Result: | Attenuated learning and memory dysfunction induced by the surgery. |
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | 4°C, sealed storage, away from moisture *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture) |
| 溶解性数据 | In Vitro: DMSO : 250 mg/mL(550.04 mM;Need ultrasonic) 配制储备液 | 1 mM | 2.2002 mL | 11.0009 mL | 22.0017 mL | | 5 mM | 0.4400 mL | 2.2002 mL | 4.4003 mL | | 10 mM | 0.2200 mL | 1.1001 mL | 2.2002 mL |
*请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。 In Vivo: 请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂： ——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶 1. 请依序添加每种溶剂： 10% DMSO 40%PEG300 5%Tween-80 45% saline Solubility: ≥ 2.08 mg/mL (4.58 mM); Clear solution
此方案可获得 ≥ 2.08 mg/mL (4.58 mM，饱和度未知) 的澄清溶液。 以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。 2. 请依序添加每种溶剂： 10% DMSO 90% (20%SBE-β-CDin saline) Solubility: ≥ 2.08 mg/mL (4.58 mM); Clear solution
此方案可获得 ≥ 2.08 mg/mL (4.58 mM，饱和度未知) 的澄清溶液。 以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。 3. 请依序添加每种溶剂： 10% DMSO 90%corn oil Solubility: ≥ 2.08 mg/mL (4.58 mM); Clear solution
此方案可获得 ≥ 2.08 mg/mL (4.58 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。 以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。 *以上所有助溶剂都可在本网站选购。
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