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Janelia Fluor 549,free acid
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Janelia Fluor 549,free acid图片
CAS NO:2245946-45-4
包装:5mg
市场价:5571元

产品介绍
Janelia Fluor 549,游离酸 (JF549 TFA) 是一种荧光染料,最大吸收 (Λab (max)) 为 549 nm,发射最大值 (Λem (max)) 为 571 nm。
Cas No.2245946-45-4
别名JF549 TFA
Canonical SMILESOC(C1=CC=C(C(C(C2=CC=C(C=C2OC3=C/4)N5CCC5)=C3C=CC4=[N+]6CCC/6)=C1)C([O-])=O)=O.FC(F)(F)C(O)=O
分子式C27H22N2O5.C2HF3O2
分子量568.5
溶解度Soluble in DMSO
储存条件Store at -20°C
General tipsFor obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
Shipping ConditionEvaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request
产品描述

Yellow fluorescent dye; supplied as a free acid. Used in protocol (2017 Grimm et al - see references below) for the synthesis of Janelia Fluor® HaloTag® and SNAP-Tag® ligands, for use in live cell imaging experiments. Also suitable for flow cytometry. Excitation maximum = 549 nm; emission maximum = 571 nm; Quantum yield = 0.88; Extinction coefficient = 101,000 M-1cm-1.

Grimm et al (2015) A general method to improve fluorophores for live-cell and single-molecule microscopy. Nat. Methods 12 244 PMID:25599551 |Grimm et al (2017) Synthesis of Janelia Fluor HaloTag and SNAP-Tag Ligands and Their Use in Cellular Imaging Experiments. Methods Mol.Biol. 1663 179 PMID:28924668