CAS NO: | 2127150-20-1 |
包装: | 2mg |
Janelia Fluor 669, SE (JF669, SE) 是一种红色荧光染料,可在温和条件下 (DIEA, DMF) 直接与可用的含硫醇 HaloTag 配体反应,一步即可得到 JF669–HaloTag 配体(Ex \u003d 669 纳米;Em \u003d 682 纳米)。
Cas No. | 2127150-20-1 |
别名 | JF669, SE; JF669, NHS |
Canonical SMILES | C[Si](C1=CC(N2CCC2)=CC=C1C(C3=C(F)C(SCC(ON4C(CCC4=O)=O)=O)=C(F)C(F)=C3C([O-])=O)=C5C=C/6)(C)C5=CC6=[N+]7CCC\7 |
分子式 | C34H30F3N3O6SSi |
分子量 | 693.77 |
溶解度 | Soluble in DMSO |
储存条件 | Store at -20°C |
General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while. |
Shipping Condition | Evaluation sample solution : ship with blue ice All other available size: ship with RT , or blue ice upon request |
产品描述 | Red fluorescent dye; supplied as an NHS ester for coupling to primary amine groups. NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag(R) and SNAP-tag(R). Suitable for confocal fluorescent imaging and super resolution microscopy (SRM) techniques, such as dSTORM (live and fixed cells). Cell permeable. Excitation maximum = 669 nm; emission maximum = 682 nm; Quantum yield = 0.37; Extinction coefficient = 116,000 M-1cm-1; Correction factor = 0.0430. Retains 97% fluorescence after 30 bleaching cycles. We also offer Janelia Fluor(R) conjugated antibodies and custom conjugation services with our sister company Novus Biologicals. HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc. Grimm et al (2017) A general method to fine-tune fluorophores for live-cell and in vivo imaging. Nat.Methods 14 987 PMID:28869757 |