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Janelia Fluor 669,SE
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Janelia Fluor 669,SE图片
CAS NO:2127150-20-1
包装:2mg

产品介绍

Janelia Fluor 669, SE (JF669, SE) 是一种红色荧光染料,可在温和条件下 (DIEA, DMF) 直接与可用的含硫醇 HaloTag 配体反应,一步即可得到 JF669–HaloTag 配体(Ex \u003d 669 纳米;Em \u003d 682 纳米)。

Cas No.2127150-20-1
别名JF669, SE; JF669, NHS
Canonical SMILESC[Si](C1=CC(N2CCC2)=CC=C1C(C3=C(F)C(SCC(ON4C(CCC4=O)=O)=O)=C(F)C(F)=C3C([O-])=O)=C5C=C/6)(C)C5=CC6=[N+]7CCC\7
分子式C34H30F3N3O6SSi
分子量693.77
溶解度Soluble in DMSO
储存条件Store at -20°C
General tipsFor obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
Shipping ConditionEvaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request
产品描述

Red fluorescent dye; supplied as an NHS ester for coupling to primary amine groups. NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag(R) and SNAP-tag(R). Suitable for confocal fluorescent imaging and super resolution microscopy (SRM) techniques, such as dSTORM (live and fixed cells). Cell permeable. Excitation maximum = 669 nm; emission maximum = 682 nm; Quantum yield = 0.37; Extinction coefficient = 116,000 M-1cm-1; Correction factor = 0.0430. Retains 97% fluorescence after 30 bleaching cycles. We also offer Janelia Fluor(R) conjugated antibodies and custom conjugation services with our sister company Novus Biologicals. HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.

Grimm et al (2017) A general method to fine-tune fluorophores for live-cell and in vivo imaging. Nat.Methods 14 987 PMID:28869757