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Hygrolidin
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Hygrolidin图片
CAS NO:83329-73-1
包装与价格:
包装价格(元)
5mg电议
10mg电议
25mg电议

产品介绍
Hygrolidin 是一种 16 元大环内酯类抗生素,来自于吸水链霉菌 (Streptomyces hygroscopicus) D-1166。Hygrolidin 对Valsa ceratosperma具有抗真菌活性。Hygrolidin 诱导 p21 表达并中止 G1 和 S 期的细胞周期进程。Hygrolidin 具有抗肿瘤活性。
生物活性

Hygrolidin is a 16-memberedmacrolideantibioticproduced byStreptomyces hygroscopicusD-1166. Hygrolidin hasanti-fungusactivity againstValsa ceratosperma. Hygrolidin induces p21 expression and abrogates cell cycle progression at G1 and S phases. Hygrolidin has antitumor activity[1][2][3].

IC50& Target

Macrolide

 

体外研究
(In Vitro)

Hygrolidin (0.1, 1 μg/ml; for 24 h) increases both G1 and S phase populations and decreases M phase population[1].
Hygrolidin (0.1, 1, 10 μg/ml; for 24 h) decreases the amounts of cdk4, cyclin D, cyclin B and increases the amounts of cyclin E and p21. Hygrolidin-induced p21 preferentially associates with cyclin A-cdk2 complex and inhibits it[1].
Hygrolidin (0.1, 1 μg/ml; for 24 h) selectively induces p21 in DLD-1 cells at mRNA level, but not in WI-38 fibroblasts[1].
Hygrolidin, for 3 days, inhibits growth of various cell lines including DLD-1 colon cancer, LNCaP prostate cancer, K562 leukemia cells, LNCaP prostate cancer, EL-4 lymphoma (IC50s = 1.0-33 ng/ml, respectively)[1].
Hygrolidin shows growth inhibition onTrypanosoma cruzi(IC50=1.1 nM),Trypanosoma brucei brucei(IC50=77 nM), andLeishmania donovani(IC50=72.5 nM)[3].

Cell Cycle Analysis[1]

Cell Line:DLD-1 cells
Concentration:0.1, 1 μg/ml
Incubation Time:For 24 hours
Result:Increased both G1 and S phase populations and decreased M phase population.

Western Blot Analysis[1]

Cell Line:DLD-1 cells
Concentration:0.1, 1, 10 μg/ml
Incubation Time:For 24 hours
Result:Decreased the amounts of cdk4, cyclin D, cyclin B and increased the amounts of cyclin E and p21.

RT-PCR[1]

Cell Line:DLD-1 or WI-38 cells
Concentration:0.1, 1 μg/ml
Incubation Time:For 24 hours
Result:Selectively induced p21 in DLD-1 cells at mRNA level, but not in WI-38 fibroblasts.
分子量

690.86

Formula

C38H58O11

CAS 号

83329-73-1

结构分类
  • Antibiotics
  • Macrolide Antibiotics
来源

Streptomyces hygroscopicus

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.