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Vacquinol-1 2HCl
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Vacquinol-1 2HCl图片
CAS NO:5428-80-8
规格:≥98%
包装与价格:
包装价格(元)
5mg电议
10mg电议
25mg电议
50mg电议
100mg电议
250mg电议
500mg电议

产品介绍
理化性质和储存条件
Molecular Weight (MW)425.78
FormulaC21H21ClN2O · 2HCl
CAS No.5428-80-8 (free base)
Storage-20℃ for 3 years in powder form
-80℃ for 2 years in solvent
Solubility (In vitro)DMSO: 70 mg/mL (164.4 mM)
Water: <1 mg/mL
Ethanol: <1 mg/mL
Solubility (In vivo) ClC1=CC=C(C2=NC(C=CC=C3)=C3C(C(O)C4NCCCC4)=C2)C=C1.[H]Cl
SynonymsNSC13316 hydrochloride; NSC 13316; NSC-13316; Vacquinol-1; Vacquinol 1; Vacquinol1 hydrochloride
实验参考方法
In Vitro

In vitro activity: Vacquinol-1 induces rapid and specific cell death of glioma cells with IC50 of 3.14 μM via membrane ruffling, cell rounding, massive macropinocytic vacuole accumulation, ATP depletion, and cytoplasmic membrane rupture.


Kinase Assay: Vacquinol-1 (also known as NSC-13316; NSC13316) is an MKK4 (mitogen-activated protein kinase kinase 4) activator with antitumor activity.


Cell Assay: Cell viability is measured using CellTiter-Glo Luminescent Cell Viability Assay according to the manufacturer’s instructions.

In VivoThe ability of vacquinol-1 to attenuate tumor progression was previously tested in a mouse model for human GBM. Vacquinol-1 or vehicle (DMSO) were intracranially administered into the site of original cell deposit 6 weeks after engraftment. Results showed that tumors were invariantly smaller, and the area of necrosis and hGFAP and hNestin immunoreactivity was significantly reduced. Moreove, only tumor cells in vacquinol-1-treated mice showed a massive LAMP1 staining.
Animal modelMice bearing U3013MG tumors
Formulation & DosageDissolved in DMSO; 15 μM or 20 mg/kg; Intracranial injection, i.p., p.o.
References

Cell. 2014 Apr 10;157(2):313-28.