您好,欢迎来到试剂信息网! [登录] [免费注册]
试剂信息网
位置:首页 > 产品库 > Chetomin
立即咨询
咨询类型:
     
*姓名:
*电话:
*单位:
Email:
*留言内容:
请详细说明您的需求。
*验证码:
 
Chetomin
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Chetomin图片
CAS NO:1403-36-7
规格:≥98%
包装与价格:
包装价格(元)
2mg电议
5mg电议
10mg电议
25mg电议
50mg电议

产品介绍
理化性质和储存条件
Molecular Weight (MW)710.87
FormulaC31H30N6O6S4
CAS No.1403-36-7
Storage-20℃ for 3 years in powder form
-80℃ for 2 years in solvent
Solubility (In vitro)DMSO: 100 mg/mL (140.7 mM)
Water: <1 mg/mL
Ethanol: 10 mg/mL (14.0 mM)
Solubility (In vivo) O=C1N(C)[C@]2(CO)C(N3[C@]1(SS2)C[C@@]4(N5C=C(C[C@]6(N(C)C7=O)SS[C@]7(CO)N(C)C6=O)C8 =C5C=CC=C8)[C@]3([H])NC9=C4C=CC=C9)=O
SynonymsNSC289491, BRN0077366; Chetomin; CTM; NSC-289491; NSC 289491
实验参考方法
In Vitro

In vitro activity: HIF-1 (hypoxia-inducible factors 1) is transcription factor which respond to changes of cellular environment oxygen. Chetomin selectively inhibits HIF-1 activities through disruption of the interaction of HIF-1 with its transcriptional coactivator p300. HIF-1 inhibition by chetomin effectively reduces hypoxia-dependent transcription and radiosensitizes hypoxic HT 1080 human fibrosarcoma cells in vitro. Chetomin attenuates the hypoxia-induced radioresistance of malignant glioma cell lines U251MG (DMF10: 1.35 and 1.18) and U343MG (DMF10: 1.78 and 1.48). Targeting of HIF-1 using chetomin abolishes the differentiation-inhibitory effect of hypoxia-inducible factor-1α.


Kinase Assay:


Cell Assay: In RT-PCR and clonogenic survival experiments, chetomin is added in a concentration of 150 nM to fully supplemented medium four hours before treatment with hypoxia. HT 1080 cells are then transferred to the hypoxic workstation (0.1% O2, 12 h) or to the well-humidified incubator (12 hours) without changing medium. HT1080 cells are thus treated for 16 hours with chetomin (150 nM) prior to radiation treatment.

In VivoAdministration of chetomin in combination with forskolin significantly suppresses malignant glioma growth in an in vivo xenograft model. Che-M(chetomin loaded micelles) dramatically inhibits embryonic angiogenesis, tumor-induced angiogenesis and tumor growth in zebrafish. In mouse model, Che-M suppresses tumor growth and prolongs the survival in the subcutaneous CT26 tumor model.
Animal modelTransgenic fish Tg(flk1:EGFP); Mouse model(BALB/c mice)
Formulation & DosageChetomin was loaded in micelles; 1 mg/mL(for zebrafish); 2 mg/kg(for mouse); s.c.
References

BMC Cancer. 2007 Nov 13;7:213; Br J Haematol. 2008 May; 141(3): 325–334; Nanoscale. 2014 Oct 21;6(20):11940-52.