28-Deoxonimbolide 是一种宁宾 (nimbin, HY-N3187) 型柠檬苦素，可以从Azadirachta indica种子提取物中分离出来。28-Deoxonimbolide 具有抗癌活性。 28-Deoxonimbolide 通过线粒体和死亡受体介导的途径诱导 HL60 细胞凋亡 (apoptosis)。
| 生物活性 | 28-Deoxonimbolide is animbin(HY-N3187) type limonoid, that can be isolated fromAzadirachta indicaseed extracts. 28-Deoxonimbolide shows anticancer activity. 28-Deoxonimbolide inducesapoptoticcell death in HL60 cells via both the mitochondrial- and the death receptor-mediated pathways[1]. |
| IC50& Target | Caspase-8 | Caspase-9 | Caspase-3 |
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体外研究
(In Vitro) | 28-Deoxonimbolide (1-100 μM，48 小时) 对 HL60 (白血病)、A549 (肺)、AZ521 (胃)、SK-BR-3 (乳腺)、CRL1579 (黑色素瘤)和 RPMI1788 (正常淋巴细胞) 显示强有力的细胞毒性[1]。
28-Deoxonimbolide (30 μM, 0-24 h) 诱导 HL60 细胞凋亡[1]。
28-Deoxonimbolide (30 μM, 0-24 h) 可增加裂解的 caspases-8、-9 和 -3 的水平[1]。
Cell Cytotoxicity Assay[1] | Cell Line: | HL60, A549, AZ521, SK-BR-3, CRL1579 cells, RPMI1788 | | Concentration: | 1-100 μM | | Incubation Time: | 48 h | | Result: | Exhibited potent cytotoxic activity against HL60 (leukemia), A549 (lung), AZ521 (stomach), SK-BR-3 (breast), and CRL1579 (melanoma) cells, with IC50values of 2.7 μM, 9.3 μM, 2.4 μM, 1.7 μM, 14.2 μM, and 2.7 μM, respectively. |
Apoptosis Analysis[1] | Cell Line: | HL60 cells | | Concentration: | 30 μM | | Incubation Time: | 8 and 24 h | | Result: | Induced early apoptosis in HL60 cells. The ratio of early apoptotic cells was increased after 8 h (10.9% vs 6.1% of negative control) and 24 h (18.7% vs 3.5% of negative control), and that of late apoptotic cells (upper right) was increased after 24 h (28.1% vs 2.9% of negative control). |
Western Blot Analysis[1] | Cell Line: | HL60 cells | | Concentration: | 30 μM | | Incubation Time: | 8 and 24 h | | Result: | Diminished the levels of procaspases-8, -9, and -3, and then increased the levels of cleaved caspases-8, -9, and -3. |
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| 来源 | - Plants
- Meliaceae
- Azadirachta indicaA. Juss.
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | Please store the product under the recommended conditions in the Certificate of Analysis. |
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