Z-LEHD-FMK

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Z-LEHD-FMK

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:	210345-04-3
包装:	1mg
市场价:	4200元

产品介绍

Z-LEHD-FMK 是一种选择性和不可逆的caspase-9抑制剂，可防止致命的再灌注损伤并减弱细胞凋亡。Z-LEHD-FMK 在大鼠脊髓损伤模型中也表现出神经保护作用。

生物活性

Z-LEHD-FMK is a selective and irreversible inhibitor ofcaspase-9, protects against lethal reperfusion injury and attenuatesapoptosis. Z-LEHD-FMK exhibits the neuroprotective effect in a rat model of spinal cord trauma^[1]^[2]^[3].

IC₅₀& Target^[1]

Caspase-9

体外研究
(In Vitro)

Z-LEHD-FMK (20 μM; pretreated for 30 min) completely protects HCT116 and 293 cells from TRAIL-induced toxicity^[1].
Z-LEHD-FMK (20 μM ; 6 h) protects normal human hepatocytes from TRAIL-induced apoptosis^[1].

Apoptosis Analysis^[1]

Cell Line:	SW480, H460, HCT116 and 293 cells
Concentration:	20 μM
Incubation Time:	Pretreated for 30 min
Result:	Protected HCT116 and 293 cells from TRAIL-induced apoptosis.

Western Blot Analysis^[1]

Cell Line:	HCT116, SW480 cells
Concentration:	20 μM
Incubation Time:	2 h
Result:	Protected procaspase 3 from cleavage in HCT116 cells but not in SW480 cells, especially at the 16-h time point.

体内研究
(In Vivo)

Z-LEHD-FMK (0.8 μmol/kg; i.v. for 7 d) protects neurons, glia, myelin, axons, and intracellular organelles in spinal cord injury (SCI) rats^[2].

Animal Model:	Male Wistar albino rats (250-350 g) with SCI^[2]
Dosage:	0.8 μmol/kg
Administration:	I.v. for 1 or 7 days
Result:	Decreased the mean apoptotic cell count at 24 hours and 7 days postinjury.

分子量

690.72

性状

Solid

Formula

C₃₂H₄₃FN₆O₁₀

CAS 号

210345-04-3

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Sealed storage, away from moisture

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据

In Vitro:

DMSO : 100 mg/mL(144.78 mM;Need ultrasonic and warming)

配制储备液

浓度溶剂体积质量

1 mg

5 mg

10 mg

1 mM	1.4478 mL	7.2388 mL	14.4776 mL
5 mM	0.2896 mL	1.4478 mL	2.8955 mL
10 mM	0.1448 mL	0.7239 mL	1.4478 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。