L67 (DNA Ligase Inhibitor) 是一种竞争性的 DNA 连接酶抑制剂，能有效抑制 DNA 连接酶 I 和 III (IC50都为 10 μM)。L67 (DNA Ligase Inhibitor) 可通过降低癌细胞线粒体 DNA 水平，增加线粒体活性氧水平，造成核 DNA 损伤。L67 (DNA Ligase Inhibitor) 还能激活癌细胞中依赖Caspase 1的凋亡通路，可用于癌症的研究。
| 生物活性 | L67 (DNA Ligase Inhibitor) is a competitiveDNA ligaseinhibitor that effectively inhibitsDNAligasesI/III(bothIC50are 10 μM). L67 (DNA Ligase Inhibitor) can cause nuclear DNA damage by reducing levels of mitochondrial DNA and increasing levels of mitochondrially-generated ROS. L67 (DNA Ligase Inhibitor) also activates theCaspase 1-dependentapoptosispathway incancercells, can be used incancerresearch[1][2]. |
体外研究
(In Vitro) | L67 (10, 15 μM; 24 h) promotes nuclear DNA damage and (0-50 μM) increases level of mSOX by inhibiting mitochondrial LigIIIα in HeLa cells[1].
L67 (10 μM; 24 h) induces changes in mitochondrial function that causes a reduction in OCR and mitochondrial DNA, and abnormal mitochondrial morphology in HeLa[1].
L67 (10, 100 μM; 24 h) induces apoptosis in cancer cells[1].
L67 (0-30 μM; 24 h) selectively induces cell death in cancer cells by activating a caspase 1-dependent apoptotic pathway[1].
Cell Viability Assay[1] | Cell Line: | HeLa cells | | Concentration: | 10, 15 μM; 0-50 μM | | Incubation Time: | 24 h | | Result: | Increased the formation of nuclear γH2AX foci and steady state levels of γH2AX when at 10 or 15 μM. (γH2AX: a sign of DNA double-strand breaks).
Resulted in a concentration (0-50 μM)-dependent increase in mSOX (mitochondrial superoxide) levels. (mSOX is a major cause of the cellular oxidative damage). |
Cell Viability Assay[1] | Cell Line: | HeLa cells | | Concentration: | 10 μM | | Incubation Time: | 24 h | | Result: | Reduced oxygen consumption rate (OCR) approximately 20%.
Resulted in about a 25% reduction in mitochondrial DNA. |
Apoptosis Analysis[1] | Cell Line: | HeLa cells | | Concentration: | 10, 100 μM | | Incubation Time: | 24 h | | Result: | Result:Induced apoptosis, and at 100 μM with apoptotic cells constituting about 50% of the HeLa cell population. |
Cell Viability Assay[1] | Cell Line: | HeLa cells | | Concentration: | 0-30 μM | | Incubation Time: | 24 h | | Result: | Activates a caspase 1-dependent cell death pathway in cancer cells. |
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | | Powder | -20°C | 3 years | | 4°C | 2 years | | In solvent | -80°C | 6 months | | -20°C | 1 month |
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| 溶解性数据 | In Vitro: DMSO : ≥ 33 mg/mL(67.89 mM) H2O : 1 mg/mL(2.06 mM;Need ultrasonic) *"≥" means soluble, but saturation unknown. 配制储备液 | 1 mM | 2.0571 mL | 10.2857 mL | 20.5715 mL | | 5 mM | 0.4114 mL | 2.0571 mL | 4.1143 mL | | 10 mM | 0.2057 mL | 1.0286 mL | 2.0571 mL |
*请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。 |
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