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Belotecan
本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。
Belotecan图片
CAS NO:256411-32-2
包装与价格：
包装价格(元)
100mg电议
250mg电议
500mg电议

产品名称
CKD-602 free base
产品介绍
Belotecan (CKD-602 free base) 是DNA topoisomerase I抑制剂。Belotecan 诱导细胞凋亡以及周期阻滞。Belotecan 是一种具有抗肿瘤效应的喜树碱衍生物，可用于癌症的研究。
生物活性

Belotecan (CKD-602 free base) is aDNAtopoisomeraseIinhibitor. Belotecan induces cellapoptosisand cell-cycle arrest. Belotecan is a camptothecin analogue with anti-tumor effects, it can be used for the research ofcancer[1].

IC50& Target

IC50: 30 ng/mL (Caski cells), 150 ng/mL (HeLa cells), 150 ng/mL (SiHa cells)[1]

体外研究
(In Vitro)

Belotecan (0-600 ng/mL; 0-72 h) time- and dose-dependently inhibits viability of Caski cells, HeLa cells and SiHa cells at 48 h with IC50values of 30, 150 and 150 ng/mL, respectively[1].
Belotecan (0-150 ng/mL; 48 h) induces cell apoptosis and cell-cycle arrest, and affects PARP, cleaved PARP, BAX, p53, Ser15, cell cycle related protein expression and cancer invasion in cervical cancer[1].

Apoptosis Analysis[1]

Cell Line:Caski, HeLa and SiHa cell lines
Concentration:0-150 ng/mL
Incubation Time:48 h
Result:Showed strong pro-apoptotic activities to Caski, HeLa and SiHa cells.

Western Blot Analysis[1]

Cell Line:Caski, HeLa and SiHa cell lines
Concentration:0-150 ng/mL
Incubation Time:48 h
Result:Increased the expression of PARP, cleaved PARP, BAX, p53, Ser15, cyclin B1, phosphorylated cyclin B1 and phospho-cdc2 (Tyr15) protein, and decreased MMP2 and VEGF protein expression.

Cell Cycle Analysis[1]

Cell Line:Caski, HeLa and SiHa cell lines
Concentration:0-150 ng/mL
Incubation Time:48 h
Result:Induced cell-cycle arrest in the G2/M phase in cervical cancer.

Cell Invasion Assay[1]

Cell Line:Caski, HeLa and SiHa cell lines
Concentration:0-150 ng/mL
Incubation Time:48 h
Result:Inhibited cancer invasion in cervical cancer.
体内研究
(In Vivo)

Belotecan (25 mg/kg; i.v. for 16 days at 4-day intervals) inhibits tumor growth in CaSki-xenografts nude mice[1].

Animal Model:BALB/c-nude mice with CaSki cells injection[1]
Dosage:25 mg/kg
Administration:Intravenous injection; 25 mg/kg; 16 days at 4-day intervals
Result:Significantly inhibited the tumor growth and showed no significant difference in bodyweight of xenograft mice and the controls.
Clinical Trial
分子量

433.50

Formula

C25H27N3O4

CAS 号

256411-32-2

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.