Gefitinib

立即咨询

Gefitinib

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:	184475-35-2
包装:	50mg, 100mg, 500mg

包装与价格：

产品名称

ZD-1839, Iressa

产品介绍

Gefitinib (ZD1839)是一种EGFR酪氨酸激酶和Akt磷酸化抑制剂，吉非替尼可以显著下调非小细胞肺癌细胞(如PC9、H1437和H1573)中CD47的表达，并增加DC的吞噬功能。吉非替尼联合应用CD47抗体可增强巨噬细胞的吞噬功能。

细胞实验
细胞系	MDA-361, SKBR-3, MDA-453 and BT-474 cells
方法	Monolayer Growth and Anchorage-independent Growth Assays. For monolayer growth, cells were seeded at a density of 3–4 104 cells in 12-well plates. Twenty-four h later, ZD1839 was added to the cells. Fresh medium ZD1839 was replaced on day 3. On day 5, cells were harvested by trypsinization and counted with a Zeiss Coulter Counter (Beckman Coulter, Miami, FL). Colony-forming assays in soft agarose were performed as described previously (30). Tumor cell colonies measuring 50 m were counted after 7 days using an Omnicon 3800 colony counter and Tumor Colony Analysis V2.IIA software (Imaging Products International, Inc.).
浓度	0.1–10 μ M
处理时间	4 days

动物实验
动物模型	BT-474 cells Xenograft in Athymic Mice
配制	0.05% Tween 80
剂量	200 mg/kg/day for 28 days
给药处理	oral gavage

动物 A (mg/kg) = 动物 B (mg/kg) ×	动物 B的K_m系数
	动物 A的K_m系数

例如，依据体表面积折算法，将化合物用于小鼠的剂量20 mg/kg 换算成大鼠的剂量，需要将20 mg/kg 乘以小鼠的K_m系数（3），再除以大鼠的K_m系数（6），得到化合物用于大鼠的等效剂量为10 mg/kg。

以下数据基于产品分子量，对于特殊产品，请参照COA中的储备液配制条件和说明进行操作。

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.2376 mL	11.1882 mL	22.3764 mL
5 mM	0.4475 mL	2.2376 mL	4.4753 mL
10 mM	0.2238 mL	1.1188 mL	2.2376 mL