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Epsilon-V1-2
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Epsilon-V1-2图片
CAS NO:182683-50-7
包装与价格:
包装价格(元)
5mg电议
10mg电议
25mg电议
50mg电议
100mg电议

产品名称
ε-V1-2
EAVSLKPT
产品介绍
Epsilon-V1-2 (ε-V1-2) 是一种 PKCε 衍生肽,是一种选择性PKCε抑制剂。Epsilon-V1-2 抑制 PKCε 的转运,但不抑制 α-,β- 和 δPKC 的转运。
生物活性

Epsilon-V1-2 (ε-V1-2), a PKCε-derived peptide, is a selectivePKCεinhibitor. Epsilon-V1-2 inhibits the translocationofPKCε, but not α-, β-, and δPKC[1].

IC50& Target

PKCε

 

体外研究
(In Vitro)

Epsilon-V1-2 (ε-V1-2), a PKCε-derived peptide containing the site for its specific receptor for activated C kinase (RACK), inhibits translocation of PKCε and reduces insulin response to glucose[1].
Epsilon-V1-2 (ε-V1-2; 1 μM, 24 hours) treatment significantly inhibits Oleic acid (OA)-induced connexin 43 (Cx43) Ser368 phosphorylation and prevents OA-induced gap junction disassembly in cardiomyocytes[2].

体内研究
(In Vivo)

Epsilon-V1-2 (20 mg/kg/day; osmotic pumps; daily; for 4 weeks) treatment significantly improves the beating score in a murine heterotopic transplantation model. Epsilon-V1-2 reduces infiltration of macrophages and T cells into the cardiac grafts, and decreases parenchymal fibrosis. Epsilon-V1-2 treatment almost abolishes the rise in pro-fibrotic cytokine, TGF-β and monocyte recruiting chemokine MCP-1 levels[3].

Animal Model:C57BL/6J mice transplanted the hearts of FVB mice[3]
Dosage:20 mg/kg/day
Administration:0.1 mL osmotic pumps implanted subcutaneously; daily; for 4 weeks
Result:Significantly improved the beating score throughout the treatment.
分子量

843.96

性状

Solid

Formula

C37H65N9O13

CAS 号

182683-50-7

Sequence Shortening

EAVSLKPT

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Sealed storage, away from moisture

Powder-80°C2 years
-20°C1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据
In Vitro: 

H2O : 50 mg/mL(59.24 mM;Need ultrasonic)

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM1.1849 mL5.9245 mL11.8489 mL
5 mM0.2370 mL1.1849 mL2.3698 mL
10 mM0.1185 mL0.5924 mL1.1849 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture)。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: PBS

    Solubility: 100 mg/mL (118.49 mM); Clear solution; Need ultrasonic

*以上所有助溶剂都可在本网站选购。