CC-90005 是一种有效,选择性和具有口服活性的蛋白激酶 C-θ (PKC-θ) 抑制剂,IC50值为 8 nM。CC-90005 显示了对 PKC-θ 的选择性超过 PKC-δ (IC50=4440 nM)。CC-90005 可以通过抑制 IL-2 表达抑制 T 细胞活化。
生物活性 | CC-90005 is a potent, selective and orally active inhibitor ofprotein kinase C-θ (PKC-θ), with anIC50of 8 nM. CC-90005 shows selectivity for PKC-θ over PKC-δ (IC50=4440 nM). CC-90005 can inhibit T cell activation by inhibitingIL-2expression[1]. |
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体外研究 (In Vitro) | CC-90005 shows the exquisite selectivity of CC-90005, with IC50s for all other family members of >3 μM[1]. CC-90005 is a moderate inhibitor of both CYP2C9 (IC50=8 μM) and CYP2C19 (IC50=5.9 μM) in human liver microsomes[1]. CC-90005 inhibits IL-2 expression in LRS_WBC human PBMCs, with an IC50of 0.15 μM[1]. CC-90005 (1-10 μM; 24 h) inhibits T cell proliferation in PBMCs by 51% at 1 μM and 88% at 3 μM[1].
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体内研究 (In Vivo) | CC-90005 (3-30 mg/kg; p.o. twice daily for 4 days) significantly reduces the popliteal lymph node (PLN) size in a model of chronic T cell activation[1]. CC-90005 (100 mg/kg; a single p.o.) significantly inhibits plasma and spleen IL-2 release by 51 and 54%, respectively[1]. CC-90005 exhibits reasonable oral bioavailability (66 and 46%) and Cmax(1.18 and 1.2 μM) following oral administration (10 and 3 mg/kg) in rat and dog, respectively[1]. CC-90005 exhibits the mean residence time (0.52 and 2.0 h), CL (69.1 and 20.5 mL/min/kg) and Vss (2.11 and 2.44 L/kg) following intravenous administration (2 and 1 mg/kg) in rat and dog, respectively[1].
Animal Model: | B6D2F1 mice (20 g) were injected with allogeneic spleen cells | Dosage: | 3, 10, 30 mg/kg | Administration: | P.o. twice daily for 4 days | Result: | Inhibited PLN size by 45 and 38% at doses of 10 and 30 mg/kg, respectively. |
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运输条件 | Room temperature in continental US; may vary elsewhere. |
储存方式 | Powder | -20°C | 3 years | | 4°C | 2 years | In solvent | -80°C | 6 months | | -20°C | 1 month |
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溶解性数据 | In Vitro: DMSO : 62.5 mg/mL(139.67 mM;Need ultrasonic) 配制储备液 1 mM | 2.2347 mL | 11.1737 mL | 22.3474 mL | 5 mM | 0.4469 mL | 2.2347 mL | 4.4695 mL | 10 mM | 0.2235 mL | 1.1174 mL | 2.2347 mL |
*请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。 In Vivo: 请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液,再依次添加助溶剂: ——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用;
以下溶剂前显示的百 分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶 1. 请依序添加每种溶剂: 10% DMSO 40%PEG300 5%Tween-80 45% saline Solubility: ≥ 1.25 mg/mL (2.79 mM); Clear solution
此方案可获得 ≥ 1.25 mg/mL (2.79 mM,饱和度未知) 的澄清溶液。 以 1 mL 工作液为例,取 100 μL 12.5 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。 2. 请依序添加每种溶剂: 10% DMSO 90% (20%SBE-β-CDin saline) Solubility: ≥ 1.25 mg/mL (2.79 mM); Clear solution
此方案可获得 ≥ 1.25 mg/mL (2.79 mM,饱和度未知) 的澄清溶液。 以 1 mL 工作液为例,取 100 μL 12.5 mg/mL 的澄清 DMSO 储备液加到 900 μL20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。 3. 请依序添加每种溶剂: 10% DMSO 90%corn oil Solubility: ≥ 1.25 mg/mL (2.79 mM); Clear solution
此方案可获得 ≥ 1.25 mg/mL (2.79 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。 以 1 mL 工作液为例,取 100 μL 12.5 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。 *以上所有助溶剂都可在本网站选购。
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