Scopolamine hydrochloride

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本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:

55-16-3

包装与价格：

包装	价格(元)
100mg	电议
250mg	电议
500mg	电议

产品名称

(-)-Scopolamine hydrochloride
HYOSCINE HYDROCHLORIDE

产品介绍

Scopolamine hydrochloride 是一种非选择性和高亲和力的毒蕈碱（muscarinic）拮抗剂。Scopolamine hydrochloride 可通过血脑屏障。Scopolamine hydrochloride 可逆地抑制5-HT3受体，IC₅₀为 2.09 μM。Scopolamine hydrochloride 可通过改变胆碱能系统诱导阿尔茨海默病样病理。

生物活性

Scopolamine hydrochloride is a non-selective and high affinity (≤1 nM)muscarinicantagonist that is used for the prevention of post-operative nausea and vomiting. Scopolamine readily passes the blood brain barrier. Scopolamine also reversibly inhibits5-HT3receptor-responses with anIC₅₀of 2.09 μM^[1]. Scopolamine induces Alzheimer's disease-like pathology through alteration of cholinergic system^[2].

IC₅₀& Target^[1]

5-HT₃Receptor

2.09 μM (IC₅₀)

体外研究
(In Vitro)

Scopolamine blocks nicotinic acetylcholine receptors (IC₅₀=928 μM) and increases the expression of α7 nACh receptors. Scopolamine displays concentration-dependent competition with 0.6 nM [³H]Granisetron, yielding an average pK_iof 5.17±0.24 (K_i=6.76 μM). Concentration-dependent competition of fluorescently labeled form of granisetron (G-FL) with Scopolamine gives an average pK_iof 5.31±0.09 (K_i=4.90 μM).Scopolamine blocks muscarinic receptors and induces a cognitive deficit^[1].
Scopolamine (0.1-3 mM; for 24 hours) induces cytotoxicity, ROS generation and apoptosis of SH-SY5Y cells^[2].

Cell Viability Assay^[2]

Cell Line:	Human neuroblastoma SH-SY5Y cell line
Concentration:	0.1-3 mM (usually 2 mM)
Incubation Time:	24 hours
Result:	Exposure to 1-3 mM for 24 hours significantly decreased human neuroblastoma SH-SY5Y cell viability in a dose-dependent manner. In all subsequent experiments, 2 M resulted in 50.6±2.2% loss in cell viability.

体内研究
(In Vivo)

Scopolamine induces memory impairment associated with attenuation of cholinergic neurotransmission, as well as an increases of processes connected with oxidative stress in the brain^[3].

Animal Model:	Naive male Swiss mice weighing 20-25^[3]
Dosage:	1 mg/kg
Administration:	Administered intraperitoneally (i.p.); to measure the memory acquisition processes, Scopolamine was administered 20 min before the pretest; to measure the memory consolidation processes, Scopolamine was administered immediately after the pretest
Result:	In scopolamine-treated group, there was a significant decrease in the index of latency (IL) value as compared with the saline-treated mice, indicating that scopolamine at the used dose impaired acquisition of memory and learning.

分子量

339.81

Formula

C₁₇H₂₂ClNO₄

CAS 号

55-16-3

结构分类

Alkaloids
Other Alkaloids

来源

Plants
Solanaceae
Atropa belladonnaLinn.

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.