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Ac-DEVD-CHO
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Ac-DEVD-CHO图片
CAS NO:169332-60-9
规格:≥98%
包装与价格:
包装价格(元)
2mg电议
5mg电议
10mg电议
25mg电议
50mg电议
100mg电议

产品介绍
理化性质和储存条件
Molecular Weight (MW) 502.47
Formula C20H30N4O11
CAS No. 169332-60-9
Storage-20℃ for 3 years in powder form
-80℃ for 2 years in solvent
Solubility (In vitro)DMSO: 10 mM
Water: 100 mg/mL (199.01 mM)
Ethanol: N/A
Solubility (In vivo) N/A
Synonyms Caspase-3 Inhibitor I, N-Ac-Asp-Glu-Val-Asp-CHO; Ac-DEVD CHO; Ac DEVD-CHO; Ac DEVD CHO
实验参考方法
In Vitro

In vitro activity: Ac-DEVD-CHO is a potent inhibitor of caspase-3 (Ki = 230 pM). In contrast, caspase-2 cleaves the tetrapeptide substrate poorly and is only weakly inhibited by this aldehyde (Ki = 1.7 μM). Group III caspases are broadly inhibited by Ac-DEVD-CHO with Ki values ranging from 1 to 300 nM. Inhibition of caspase-3 by Ac-DEVD-CHO in isolated working-heart rat model significantly improves post-ischemic contractile recovery of stunned myocardium, even when given after the onset of ischemia. The mechanism(s) of protection by Ac-DEVD-CHO appear to be independent of apoptosis. Troponin I cleavage was not inhibited by Ac-DEVD-CHO.


Kinase Assay: Ac-DEVD-CHO is a potent inhibitor of caspase-3 (Ki = 230 pM). In contrast, caspase-2 cleaves the tetrapeptide substrate poorly and is only weakly inhibited by this aldehyde (Ki = 1.7 μM). Group III caspases are broadly inhibited by Ac-DEVD-CHO with Ki values ranging from 1 to 300 nM.


Cell Assay: Apaf-1–/– cells were treated with the indicated concentration of Ac-DEVD-CHO for 2 h prior to treatment with 4 μM CHX alone or with TNF for 6 h. LEHDase and DEVDase activities were standardized to the lactate dehydrogenase activity in the sample and are presented as percentages of the activity in TNF-plus-CHX-treated cells not treated with Ac-DEVD-CHO (0 μM).

In Vivo Ac-DEVD-CHO administered at the time of MI results in a 61% reduction in activated caspase-3 expression in cardiomyocytes (p<0.05), and an 84% reduction in cardiomyocyte apoptosis in the young animals. However, in the aging mice, caspase inhibition had no effect on activated caspase-3 expression or cardiomyocyte apoptosis. Ac-DEVD-CHO suppressed and/or delayed the progression of photoreceptor cell damage in rats and delays disease progression in rd gene-carring mice, which normally develop retinal degeneration early in life.
Animal modelC57Bl6 mice.
Formulation & Dosage3 mg/kg; i.p.
ReferencesJ Biol Chem. 1998 Dec 4;273(49):32608-13; J Am Coll Cardiol. 2001 Dec;38(7):2063-70; Mol Cell Biol. 2006 Nov;26(21):7880-91. Epub 2006 Sep 11.