YS-49 monohydrate

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YS-49 monohydrate

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

包装与价格：

包装	价格(元)
10mg	电议
50mg	电议

产品介绍

YS-49 (monohydrate) 是PI3K/Akt(RhoA 的下游靶标) 的激活剂，可减少 3-甲基胆碱处理的细胞中 RhoA/PTEN 的激活。YS-49 能通过诱导血红素加氧酶 (HO-1) 来抑制血管紧张素 II (Ang II)刺激 VSMC 细胞的增殖。YS-49 是异喹啉化合物生物碱，因能激活心脏β-adrenoceptors而具有强烈的正性肌力作用。

生物活性

YS-49 (monohydrate) is aPI3K/Akt(a downstream target of RhoA) activator, to reduce RhoA/PTEN activation in the 3-methylcholanthrene-treated cells. YS-49 inhibitsangiotensin II (Ang II)-stimulated proliferation of VSMCs via induction of heme oxygenase (HO)-1. YS-49 is also an isoquinoline compound alkaloid, has a strong positive inotropic action through activation of cardiacβ-adrenoceptors^[1]^[2]^[3].

IC₅₀& Target

PI3K/Akt^[3]

体外研究
(In Vitro)

YS-49 (1-100 μM; 18 hours; RAVSMC and RAW 264.7 cells) concentration-dependently inhibits the accumulation of nitrite in both RAVSMC and RAW 264.7 exposed to lipopolysaccharide (LPS) plus INF-γ, withIC₅₀values of 22 μM and 30 μM, respectively^[2].
YS-49 (10-100 μM; 18 hours; RAVSMC and RAW 264.7 cells) suppresses iNOS gene expression induced by LPS and/or cytokines in RAVSMC and RAW 264.7 cells at the transcriptional level^[2].

Cell Viability Assay^[2]

Cell Line:	RAVSMC and RAW 264.7 cells
Concentration:	10 μΜ, 30 μΜ and 100 μΜ(RAVSMC); 1 μΜ, 10 μΜ and 100 μM (RAW 264.7)
Incubation Time:	18 hours
Result:	Inhibited the accumulation of nitrite in both RAVSMC and RAW 264.7 exposed to LPS+INF-γ, withIC₅₀values of 22 and 30 μM, respectively.

Western Blot Analysis^[2]

Cell Line:	RAVSMC and RAW 264.7 cells
Concentration:	10 μΜ, 30 μΜ and 100 μΜ
Incubation Time:	18 hours
Result:	Concentration-dependently inhibited the expression of iNOS protein induced by LPS plus IFN-γ.

体内研究
(In Vivo)

YS-49 (5 mg/kg; intraperitoneal injection; 8 hours; male Sprague Dawley rats) treatment significantly reduces serum NOx levels in LPS-treated rats, the NOx levels reduce from 86 μM to 34 μM^[2].

Animal Model:	Male Sprague Dawley rats (250-300 g)^[2]
Dosage:	5 mg/kg
Administration:	Intraperitoneal injection; 8 hours
Result:	Serum NOx levels were significantly reduced.

分子量

404.30

性状

Solid

Formula

C₂₀H₂₂BrNO₃

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)