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Calcitonin Gene Related Peptide(CGRP)II,rat
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
CAS NO:99889-63-1
包装与价格:
包装价格(元)
100mg电议
250mg电议
500mg电议

产品介绍
Calcitonin Gene Related Peptide (CGRP) II, rat,一种 CGRP 受体 (CGRP receptors) 激活剂,是一种有效且持久的血管扩张剂。Calcitonin Gene Related Peptide (CGRP) II, rat 可用于心血管疾病的研究。
生物活性

CalcitoninGene Related Peptide (CGRP) II, rat, aCGRP receptoractivator, is a potent and long-lastingvasodilator.CalcitoninGene Related Peptide (CGRP) II can be used in the research of cardiovascular diseases[1].

体外研究
(In Vitro)

Calcitonin Gene Related Peptide (CGRP) II, rat induces vasodilator effects in small-diameter pig left anterior descending (LAD) coronary arteries with an EC50value of 0.56 nM[1].
Calcitonin Gene Related Peptide (CGRP) II, rat relaxes the spontaneous tone in isolated internal anal sphincter (IAS) strips, with an EC50value of 83 μM[2].
Calcitonin Gene Related Peptide (CGRP) II, rat (rβCGRP) inhibits [125I]hαCGRP binding (IC50: 7 nM), and stimulates cAMP accumulation (EC50: 0.56 nM) in the cells co-expressing mRAMP1 (mouse receptor-activity-modifying-protein 1) and rCRLR (rat calcitonin receptor-like receptor)[3].
Calcitonin Gene Related Peptide (CGRP) II, rat (rat-βCGRP, 0.01-100 nM) induces endothelium-independent relaxations in the coronary arteries from male and female Sprague-Dawley rats, with an IC50value of 2.8 nM approximately[4].

体内研究
(In Vivo)

Calcitonin Gene Related Peptide (CGRP) II, rat (rat-βCGRP, bolus injection, 0.3 μg/kg, at 10 min intervals) induces hypotension and vasodilatation in Male Sprague-Dawley rats[5].

Animal Model:Male Sprague-Dawley rats[5].
Dosage:0.01 ng/kg-3 μg/kg (100 μL), succeeded by a 150 μL isotonic saline flush, at 10 min intervals.
Administration:A bolus injection
Result:Induced PA (pial artery) dilatation and increased in LCBFFlux(local cortical cerebral blood flow).
分子量

3805.31

Formula

C163H267N51O50S2

CAS 号

99889-63-1

Sequence

Ser-Cys-Asn-Thr-Ala-Thr-Cys-Val-Thr-His-Arg-Leu-Ala-Gly-Leu-Leu-Ser-Arg-Ser-Gly-Gly-Val-Val-Lys-Asp-Asn-Phe-Val-Pro-Thr-Asn-Val-Gly-Ser-Lys-Ala-Phe-NH2 (Disulfide bridge: Cys2-Cys7)

Sequence Shortening

SCNTATCVTHRLAGLLSRSGGVVKDNFVPTNVGSKAF-NH2 (Disulfide bridge: Cys2-Cys7)

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent & Solubility
In Vitro: 

H2O

Peptide Solubility and Storage Guidelines:

1.  Calculate the length of the peptide.

2.  Calculate the overall charge of the entire peptide according to the following table:

 ContentsAssign value
Acidic amino acidAsp (D), Glu (E), and the C-terminal -COOH.-1
Basic amino acidArg (R), Lys (K), His (H), and the N-terminal -NH2+1
Neutral amino acidGly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q)0

3.  Recommended solution:

Overall charge of peptideDetails
Negative (<0)1.  Try to dissolve the peptide in water first.
2.  If water fails, add NH4OH (<50 μL).
3.  If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (>0)1.  Try to dissolve the peptide in water first.
2.  If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.  If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0)1.  Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.  For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.