CHPG

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CHPG

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:

170846-74-9

包装与价格：

包装	价格(元)
5mg	电议
10mg	电议
50mg	电议

产品介绍

CHPG 是一个选择性的mGluR5激动剂，并且通过TSG-6/NF-κB途径减弱 BV2 小神经胶质细胞 SO₂诱导的氧化应激和炎症。 CHPG 通过ERK和Akt途径在体外和体内预防创伤性脑损伤 (TBI)。

生物活性

CHPG is a selectivemGluR5agonist, and attenuates SO₂-induced oxidative stress and inflammation throughTSG-6/NF-κBpathway in BV2 microglial cells^[1]. CHPG protects against traumatic brain injury (TBI) in vitro and in vivo by activation of theERKandAktsignaling pathways^[2].

IC₅₀& Target

mGlu₅

NF-κB

ERK

体外研究
(In Vitro)

CHPG (10-500 μM; 24 hours) significantly increases the cell viability and decreases the LDH release after SO₂derivatives treatment^[1].
CHPG (0.5 mM; 30 mins ) protects BV2 cells against SO₂-induced apoptosis^[1].
CHPG (0.5 mM; 30 mins) treatment alone increases the expression of TSG-6 in both mRNA and protein levels^[1].

Cell Viability Assay^[1]

Cell Line:	BV2 microglial cells
Concentration:	10, 50, 100 and 500 μM
Incubation Time:	24 hours
Result:	Increased the cell viability.

Apoptosis Analysis^[1]

Cell Line:	BV2 microglial cells
Concentration:	0.5 mM
Incubation Time:	30 mins
Result:	Protected BV2 cells against SO₂-induced apoptosis.

Western Blot Analysis^[1]

Cell Line:	BV2 microglial cells
Concentration:	0.5 mM
Incubation Time:	30 mins
Result:	Increased the expression of TSG-6 in both mRNA and protein levels.

体内研究
(In Vivo)

CHPG (injection; 250 nM; for 7 days) reduces significantly cerebral lesion volume^[2].

Animal Model:	Adult Sprague-Dawley male rats weighing 280-320 g^[2]
Dosage:	250 nM
Administration:	Injection; for 7 days
Result:	Reduced significantly cerebral lesion volume.

分子量

201.61

性状

Solid

Formula

C₈H₈ClNO₃

CAS 号

170846-74-9

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

溶解性数据

In Vitro:

1M NaOH : 25 mg/mL(124.00 mM;ultrasonic and adjust pH to 11 with NaOH)

DMSO :< 1 mg/mL (ultrasonic)(insoluble or slightly soluble)

配制储备液

浓度溶剂体积质量

1 mg

5 mg

10 mg

1 mM	4.9601 mL	24.8004 mL	49.6007 mL
5 mM	0.9920 mL	4.9601 mL	9.9201 mL
10 mM	0.4960 mL	2.4800 mL	4.9601 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。