Nrf2/HO-1 activator 2 (compound 13m) 是二氟取代衍生物，是一种有效的Nrf2/HO-1激活剂。Nrf2/HO-1 activator 2 通过 PC12 细胞中 ERK1/2、JNK 或 Akt 的磷酸化介导 Nrf2/HO-1 通路具有神经保护和抗氧化作用。Nrf2/HO-1 activator 2 可用于帕金森病 (PD) 的研究。
| 生物活性 | Nrf2/HO-1 activator 2 (compound 13m), difluoro-substituted derivative, is a potentNrf2/HO-1activator. Nrf2/HO-1 activator 2 has neuroprotective and antioxidant effects through the Nrf2/HO-1 pathway mediated by phosphorylation of ERK1/2,JNK, orAktin PC12 cells. Nrf2/HO-1 activator 2 can be used in the research of Parkinson's disease (PD)[1]. |
体外研究
(In Vitro) | Nrf2/HO-1 activator 2 (compound 13m; 0.1-30 μM; 24 h) has protection to PC12 cells against toxicity and inhibits 6-OHDA- andRotenone(HY-B1756)- induced cell death[1].
Nrf2/HO-1 activator 2 (1-100 μM; 11 h) inhibits 6-OHDA- or rotenone-induced production of reactive oxygen species and partially attenuates lipid peroxidation in rat brain homogenates. Nrf2/HO-1 activator 2 inhibits the production of lipid peroxide in rat brain homogenates by 28.8% at 100 μM[1].
Nrf2/HO-1 activator 2 (1-100 μM; 11 h) up-regulates heme oxygenase-1 (HO-1) and Nrf2 expression[1].
Nrf2/HO-1 activator 2 (10 μM; 2-3 h; PC12 cells) activator 2 activates Nrf2/HO-1 signaling by phosphorylated ERK1/2, JNK, and Akt[1].
Cell Viability Assay[1] | Cell Line: | PC12 cells | | Concentration: | 3, 5, 7, 10 and 30 μM, 6-OHDA (50 μM); 0.1, 0.3, 1, 3, 10, and 30 μM,Rotenone(1 μM) | | Incubation Time: | 24 hours | | Result: | Exhibited neuroprotective effects on 6-OHDA-induced toxicity in dose-dependent manners.
Rescued cells from rotenone-induced damage, with increased levels of cell viability up to 79.9% at 30 μM. |
Western Blot Analysis[1] | Cell Line: | PC12 cells | | Concentration: | 0, 1, 3, 10, and 30 μM | | Incubation Time: | 24 hours | | Result: | Upregulated HO-1 levels in a dose-dependent manner, with 1.5- and 2-fold increases at 30 μM concentrations, respectively.
Enhanced the nuclear translocation of Nrf2 in PC12 cells after 3 h at 10 μM. |
Western Blot Analysis[1] | Cell Line: | PC12 cells | | Concentration: | 10 μM | | Incubation Time: | 2-3 hours | | Result: | Induced the phosphorylation of ERK1/2, JNK, and Akt. |
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | Please store the product under the recommended conditions in the Certificate of Analysis. |
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