L-ascorbic acid 2-phosphate (2-Phospho-L-ascorbic acid) magnesium hydrate is a long-actingvitamin C derivativethat can stimulatecollagen formationand expression. L-ascorbic acid 2-phosphate magnesium hydrate can be used as a culture medium supplement for theosteogenic differentiationof human adipose stem cells (hASCs). L-ascorbic acid 2-phosphate magnesium hydrate increasesalkaline phosphatase(ALP)activity and expression of runx2A in hASCs during the osteogenic differentiation^[1][2][3].

L-Ascorbic acid 2-phosphate (0.1-1.5 mM; 2 to 3 weeks with medium exchange every 2 to 3 days) magnesium hydrate significantly stimulates cell growth, whereas addition of l-Ascorbic acid (Asc) achieves only weak growth stimulation. A combination of Asc-2P and bFGF significantly increases cell growth, but supplementation with EGF and/or insulin does not have any additional effect^[1].
L-Ascorbic acid 2-phosphate (50 μM-250 μM) magnesium hydrate is needed for the effective osteogenic differentiation of human adipose stem cells (hASCs), and higher concentrations of AsA2-P results in increased runx2 expression and ALP activity. The highest proliferation, ALP activity and runx2 expression is achieved with 150 μM AsA2-P and 10 nM dexamethasone (Dex), and 250 μM AsA2-P and 5 nM Dex^[3].

C₆H₉O₉P.xH₂O.3/2Mg

1713265-25-8

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