您好,欢迎来到试剂信息网! [登录] [免费注册]
试剂信息网
位置:首页 > 产品库 > Hoechst 34580 tetrahydrochloride
立即咨询
咨询类型:
     
*姓名:
*电话:
*单位:
Email:
*留言内容:
请详细说明您的需求。
*验证码:
 
Hoechst 34580 tetrahydrochloride
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Hoechst 34580 tetrahydrochloride图片
包装与价格:
包装价格(元)
5mg电议
10mg电议
50mg电议
100mg电议
200mg电议
500mg电议

产品名称
HOE 34580 tetrahydrochloride
产品介绍
Hoechst 34580 tetrahydrochloride是一种细胞渗透性的荧光染料,用于DNA和核染色。
生物活性

Hoechst 34580 tetrahydrochloride is a cell-permeablefluorescent dyefor staining DNA and nuclei.

IC50& Target

Amyloid-β[1]

体外研究
(In Vitro)

Hoechst 34580 prefers to locate near hydrophobic regions with binding affinity with the inhibition constant IC50of 0.86±0.05 μM for Hoechst 34580. Hoechst 34580 is a good candidate for treating the Alzheimer’s disease by inhibiting Aβ formation. 50 μM Aβ42 solutions co-incubated with 100, 25, 12.5, 3.125, 0.78, and 0.1, 0.01 μM Hoechst 34580 at 37 ℃ for 70 h. Hoechst 34580 can inhibit the aggregation of Aβ42 in a dose-dependent manner. And the half-maximal concentration (IC50) is examined. The IC50is obtained by measuring the concentration of Hoechst 34580 while maintaining the Aβ42 concentration which gave 0.86±0.05 μM for Hoechst 34580[1].

分子量

597.41

性状

Solid

Formula

C27H33Cl4N7

Emission (Em)
Em 450-495 nm Blue
Excitation (Ex)
Ex 450-495 nm Blue
运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

溶解性数据
In Vitro: 

H2O : 2.67 mg/mL(4.47 mM;Need ultrasonic)

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM1.6739 mL8.3695 mL16.7389 mL
5 mM---------
10 mM---------
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture and light)。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: PBS

    Solubility: 6.25 mg/mL (10.46 mM); Clear solution; Need ultrasonic

*以上所有助溶剂都可在本网站选购。
染色示例
  • Description: Hoechst 34580 tetrahydrochloride can be used to stain nuclei, and to quantify the amount of elastin in the medial layer of the tissues.
    Method: For cell staining.
    1. Incubate cells with Hoechst 34580 tetrahydrochloride (1 μg/mL; 30 min; room temperature).
    2. Wash cells for three times with 1xPBS, sections are mounted using ProLong Gold antifade.
    3. Use a Nikon Eclipse Ti confocal microscope for image.