Hoechst 34580 tetrahydrochloride is a cell-permeablefluorescent dyefor staining DNA and nuclei.

Amyloid-β^[1]

Hoechst 34580 prefers to locate near hydrophobic regions with binding affinity with the inhibition constant IC₅₀of 0.86±0.05 μM for Hoechst 34580. Hoechst 34580 is a good candidate for treating the Alzheimer’s disease by inhibiting Aβ formation. 50 μM Aβ42 solutions co-incubated with 100, 25, 12.5, 3.125, 0.78, and 0.1, 0.01 μM Hoechst 34580 at 37 ℃ for 70 h. Hoechst 34580 can inhibit the aggregation of Aβ42 in a dose-dependent manner. And the half-maximal concentration (IC₅₀) is examined. The IC₅₀is obtained by measuring the concentration of Hoechst 34580 while maintaining the Aβ42 concentration which gave 0.86±0.05 μM for Hoechst 34580^[1].

597.41

Solid

C₂₇H₃₃Cl₄N₇

Room temperature in continental US; may vary elsewhere.

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

H₂O : 2.67 mg/mL(4.47 mM;Need ultrasonic)

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture and light)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

• 1.

  请依序添加每种溶剂： PBS

  Solubility: 6.25 mg/mL (10.46 mM); Clear solution; Need ultrasonic

• 
  Description: Hoechst 34580 tetrahydrochloride can be used to stain nuclei, and to quantify the amount of elastin in the medial layer of the tissues.
  Method: For cell staining.
  1. Incubate cells with Hoechst 34580 tetrahydrochloride (1 μg/mL; 30 min; room temperature).
  2. Wash cells for three times with 1xPBS, sections are mounted using ProLong Gold antifade.
  3. Use a Nikon Eclipse Ti confocal microscope for image.