Glycycoumarin 是一种有效的抗痉挛剂。Glycycoumarin 是甘草的主要生物活性香豆素，具有抗痉挛活性。Glycycoumarin 还具有保肝作用。Glycycoumarin 可用于腹痛和肝脏疾病的研究。
| 生物活性 | Glycycoumarin is a potentantispasmodicagent. Glycycoumarin is a major bioactive coumarin of licorice and exhibits antispasmodic activity. Glycycoumarin also has hepatoprotective effect. Glycycoumarin can be used for the research of abdominal pain and liver diseases[1][2]. |
体外研究
(In Vitro) | Glycycoumarin, (25 μM; 24 h) combining with ABT-737 (12.5 μM) synergistically, induces cell death in multiple types of liver cancer cell HepG2[2].
Glycycoumarin is highly effective against alcoholic liver disease, nonalcoholic fatty liver disease, acetaminophen-induced hepatotoxicity, and liver cancer through mechanisms involved in activation of Nrf2 antioxidant system, stimulation of AMPK-mediated energy homeostasis, induction of autophagy degradation process, and inhibiting oncogenic kinase T-lymphokine-activated killer cell-originated protein kinase activity[3].
Cell Viability Assay[2] | Cell Line: | HepG2, SMMC-7721, and Huh-7 | | Concentration: | 10, 20, 25, 30, and 40 μM | | Incubation Time: | 24 hours | | Result: | Induced cell death in multiple types of liver cancer cell lines in a dose-dependent manner. |
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体内研究
(In Vivo) | Glycycoumarin (30 μM-0.3 nM; 5 min) has an inhibitory effect on smooth muscle contraction induced by various types of stimulants through the inhibition of PDEs, especially isozyme 3, followed by the accumulation of intracellular cAMP[1].
Glycycoumarin (10 mg/kg; i.p.; once daily for 4 d) enhances tumor growth inhibition in HepG2 xenograft model in mice and shows synergistical effect withABT-737(HY-50907)[2].
| Animal Model: | Male ICR mice (aged 6 weeks, weight 25-30 g)[1] | | Dosage: | 30 μM-0.3 nM, 5 min | | Administration: | | | Result: | Inhibited the contraction induced by various types of stimulants, such as CCh, KCI, BaCI(2), and A23187 (calcium ionophore III).
Enhanced the relaxation induced by forskolin on CCh-evoked contraction and also enhances the relaxation effect of rolipram.
Associated with dose-dependent accumulation of cAMP. |
| Animal Model: | HepG2 cancer cells xenograft model in male BALB/c athymic nude mice (6-7 weeks old)[2] | | Dosage: | 10 mg/kg | | Administration: | Intraperitoneal injection; once daily for 4 days; ABT-737 group was given 100 mg/kg i.p. | | Result: | Inhibited tumor growth and resulted a reduction of the final tumor weight by 17%. |
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| 性状 | |
| Formula | |
| CAS 号 | |
| 中文名称 | |
| 结构分类 | - Phenylpropanoids
- Coumarins
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| 来源 | - Plants
- Leguminosae
- Glycyrrhiza uralensisFisch.
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | | Powder | -20°C | 3 years | | 4°C | 2 years | | In solvent | -80°C | 6 months | | -20°C | 1 month |
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| 溶解性数据 | In Vitro: DMSO : 250 mg/mL(678.65 mM;Need ultrasonic) 配制储备液 | 1 mM | 2.7146 mL | 13.5729 mL | 27.1459 mL | | 5 mM | 0.5429 mL | 2.7146 mL | 5.4292 mL | | 10 mM | 0.2715 mL | 1.3573 mL | 2.7146 mL |
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储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。 In Vivo: 请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂： ——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶 1. 请依序添加每种溶剂： 10% DMSO 40%PEG300 5%Tween-80 45% saline Solubility: ≥ 2.08 mg/mL (5.65 mM); Clear solution
此方案可获得 ≥ 2.08 mg/mL (5.65 mM，饱和度未知) 的澄清溶液。 以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。 2. 请依序添加每种溶剂： 10% DMSO 90%corn oil Solubility: 2.08 mg/mL (5.65 mM); Suspended solution; Need ultrasonic
此方案可获得 2.08 mg/mL (5.65 mM) 的均匀悬浊液，悬浊液可用于口服和腹腔注射。 以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。 *以上所有助溶剂都可在本网站选购。
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