Chicoric acid (Cichoric acid) 是一种口服有效的二咖啡基酒石酸，可以诱导活性氧 (ROS) 的产生。Chicoric acid 抑制细胞活力，并通过 ROS 介导的 PI3K/Akt 和 MAPK 信号通路诱导 3T3-L1 前脂肪细胞中的线粒体依赖性细胞凋亡。Chicoric acid 增加葡萄糖摄取，改善胰岛素抵抗，并减轻氨基葡萄糖诱导的炎症。Chicoric acid 具有抗糖尿病和抗氧化、抗炎作用。
| 生物活性 | Chicoric acid (Cichoric acid), an orally active dicaffeyltartaric acid, inducesreactive oxygen species(ROS)generation. Chicoric acid inhibits cell viability and induces mitochondria-dependentapoptosisin 3T3-L1 preadipocytes through ROS-mediated PI3K/Akt and MAPK signaling pathways. Chicoric acid increases glucose uptake, improvesinsulinresistance, and attenuates glucosamine-induced inflammation. Chicoric acid has antidiabetic properties and antioxidant, anti-inflammatory effects[1][2][3]. |
体外研究
(In Vitro) | Chicoric acid (Cichoric acid; 10-200 μM; for 24, 48, and 72 h) causes a dose- and time-dependent decrease in cell viability[1].
Chicoric acid (100 μM; 48 h) induces apoptosis through caspase-3-dependent pathway[1].
Chicoric acid (100 μM; 48 h) decreases the protein level of p-Akt[1].
Chicoric acid (25, 50, 100 μM; for 24 hours) dramatically improves glucose uptake in a dose-dependent manner, and Chicoric acid further enhances insulin-induced (100 nM; 30 min) glucose uptake by 57.7% in HepG2 cells[2].
Chicoric acid (100 μM; for 24 hours) restores glucosamine-induced impairment of GLUT2 translocation through activating PI3K/Akt pathway in HepG2 cells[2].
Chicoric acid (100 μM) has no effects on HepG2 cell viability[2].
Cell Viability Assay[1] | Cell Line: | 3T3-L1 preadipocytes | | Concentration: | 10-200 μM | | Incubation Time: | 24, 48, and 72 hours | | Result: | Had no effect on the viability of 3T3-L1 preadipocytes with 10-50 μM for 24 h, but significantly decreased cell viability with 100 μM and 200 μM. |
Apoptosis Analysis[1] | Cell Line: | 3T3-L1 preadipocytes | | Concentration: | 100 μM | | Incubation Time: | 48 hours | | Result: | Demonstrated typical characteristics of apoptosis such as cell shrinkage, chromatin condensation, and the increased permeability of cell membranes after DAPI and AO/EB staining. |
Western Blot Analysis[1] | Cell Line: | 3T3-L1 preadipocytes | | Concentration: | 100 μM | | Incubation Time: | 48 hours | | Result: | Decreased the protein level of p-Akt in a dose- and time-dependent manner.
The protein level of total Akt was not affected |
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体内研究
(In Vivo) | Chicoric acid (Cichoric acid; 60 mg/kg/day; drinking water for 4 weeks) inhibits pancreas apoptosis and adjusts islet function in diabetic mice, leading to an increase in insulin generation and secretion in C57BL/6J mice with Streptozotocin (STZ; 50 mg/kg; ip; for consecutive 5 days)[3].
| Animal Model: | C57BL/6J mice with STZ (50 mg/kg; ip; for consecutive 5 days)[3] | | Dosage: | 60 mg/kg | | Administration: | Drinking water; daily; for 4 weeks | | Result: | Inhibited pancreas apoptosis and adjusted islet function in diabetic mice, leading to an increase in insulin generation and secretion.
Regulated mitochondrial biogenesis, glycogen synthesis, and inhibited inflammation via activating antioxidant responses.
Showed a remarkable increase in body weight starting at week 7. |
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| 结构分类 | - Phenylpropanoids
- Other Phenylpropanoids
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | | Powder | -20°C | 3 years | | 4°C | 2 years | | In solvent | -80°C | 6 months | | -20°C | 1 month |
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| 溶解性数据 | In Vitro: H2O : 100 mg/mL(210.81 mM;Need ultrasonic) DMSO : 1 mg/mL(2.11 mM;Need ultrasonic) 配制储备液 | 1 mM | 2.1081 mL | 10.5403 mL | 21.0806 mL | | 5 mM | 0.4216 mL | 2.1081 mL | 4.2161 mL | | 10 mM | 0.2108 mL | 1.0540 mL | 2.1081 mL |
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储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。 In Vivo: 请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂： ——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百
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