Crosstide

立即咨询

Crosstide

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:

171783-05-4

包装与价格：

包装	价格(元)
1mg	电议
5mg	电议
10mg	电议
50mg	电议

产品介绍

Crosstide 是一种糖原合成酶激酶α/β融合蛋白序列的模拟肽，是Akt的底物。

生物活性

Crosstide is a peptide analog of glycogen synthase kinase α/β fusion protein sequence which is a substrate forAkt.

体外研究
(In Vitro)

16HBE14o– cells exposed to RV39 demonstrate a crosstide kinase activity in vitro. Serine phosphorylation of crosstide is confirmed by immunoblotting, and phosphorylation is blocked by PP2 but not by PP3^[1]. The wildtype GST-AKT2 shows significant phosphoryl transferase activity towards crosstide, reaching an initial velocity of 16 pmol phosphate/min/μg enzyme. The mutant GST-AKT2T/E,S/D displayed an initial velocity of 85 pmol phosphate/min/μg kinase for phosphorylation of Crosstide, corresponding to a 5-fold increase compared to the wildtype enzyme^[2].

分子量

1164.23

性状

Solid

Formula

C₄₈H₇₇N₁₇O₁₇

CAS 号

171783-05-4

Sequence

Gly-Arg-Pro-Arg-Thr-Ser-Ser-Phe-Ala-Glu-Gly

Sequence Shortening

GRPRTSSFAEG

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Sealed storage, away from moisture

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据

In Vitro:

H₂O : ≥ 50 mg/mL(42.95 mM)

*"≥" means soluble, but saturation unknown.

配制储备液

浓度溶剂体积质量

1 mg

5 mg

10 mg

1 mM	0.8589 mL	4.2947 mL	8.5894 mL
5 mM	0.1718 mL	0.8589 mL	1.7179 mL
10 mM	0.0859 mL	0.4295 mL	0.8589 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。