产品介绍 目录号
Serial | SCSP-514 | 标识符
Identifier | CSTR:19375.09.3101HUMSCSP514 | 描述
Description | 人结直肠癌细胞LoVo构建于1971年，来源于一名56岁的白人男性结直肠癌患者转移到左锁骨位置的一个肿瘤结节。CSAp和结肠抗原3(colon antigen 3)表达阴性。肿瘤基因c-myc、K-ras、H-ras、 N-ras、Myb, sis和fos表达阳性，但未检测到 N-myc表达。该细胞表达肿瘤特定的核基质蛋白CC-3和CC-4。每个批次均通过本库支原体检测，结果阴性。经本库STR检测结果无误，STR结果如下：D5S818: 11,13；D13S317: 8,11；D7S820:9.3,10,11；D16S539: 9,12；vWA: 17,18；THO1: 9.3；Amelogenin: X,Y；TPOX: 8,9；CSF1PO: 10,11,13,14 | 动物种别
Organism | 人；白人 | 性别
Gender | 男性，56岁 | 组织来源
Tissue and Cell Type | 结肠；分离自转移灶：左锁骨上区域 | 形态
Morphology | 上皮样，贴壁生长 | 培养基和添加剂
Complete Growth Medium and Culture Conditions | 完全培养液配方(100 ml)
Ham's F-12K (Kaighn's) Medium (Invitrogen, 21127-022) 90 ml
FBS (Gibco) 10 ml
参考传代周期：2-3天
参考传代比例：1:3-1:5
参考换液频率：每周2-3次
冻存液配方：完全培养液95%，DMSO 5%
气相：空气，95%；二氧化碳，5%。温度：37摄氏度。 | 供应限制
Permits and Restrictions | A。仅供研究之用。 | | REFERENCE | Drewinko B, et al. Further biologic characteristics of a human carcinoembryonic antigen-producing colon carcinoma cell line. J. Natl. Cancer Inst. 61: 75-83, 1978. PubMed: 276641Drewinko B, Yand LY. Restriction of CEA synthesis to the stationary phase of growth of cultured human colon carcinoma cells. Exp. Cell Res. 101: 414-416, 1976. PubMed: 964319Drewinko B, et al. Establishment of a human carcinoembryonic antigen-producing colon adenocarcinoma cell line. Cancer Res. 36: 467-475, 1976. PubMed: 1260746Trainer DL, et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874Keesee SK, et al. Nuclear matrix proteins in human colon cancer. Proc. Natl. Acad. Sci. USA 91: 1913-1916, 1994. PubMed: 8127905Drewinko B, et al. Response of exponentially growing, stationary-phase, and synchronized cultured human colon carcinoma cells to treatment with nitrosourea derivatives. Cancer Res. 39: 2630-2636, 1979. PubMed: 445465Miranda L, et al. Isolation of the human PC6 gene encoding the putative host protease for HIV-1 gp160 processing in CD4+ T lymphocytes. Proc. Natl. Acad. Sci. USA 93: 7695-7700, 1996. PubMed: 8755538 |
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