产品介绍 目录号
Serial | SCSP-531 | 标识符
Identifier | CSTR:19375.09.3101HUMSCSP531 | 描述
Description | 人乳腺癌细胞MCF7保留了分化乳腺上皮的许多特性。包括：能通过胞质雌激素受体加工雌二醇并能形成圆形复合物(domes)。该细胞含有WNT7B癌基因。肿瘤坏死因子α(TNF alpha)可以抑制MCF-7细胞生长。细胞经抗雌激素处理后能调节胰岛素样生长因子结合蛋白IGFBPS分泌。每个批次均通过本库支原体检测，结果为阴性。经本库STR检测结果无误。STR结果如下：D5S818: 11,12；D13S317: 11；D7S820: 8,9；D16S539: 11,12；vWA: 14,15；THO1: 6；Amelogenin: X；TPOX: 9,12；CSF1PO: 10 | 动物种别
Organism | 人；白人 | 性别
Gender | 女性，69岁 | 组织来源
Tissue and Cell Type | 乳腺、胸；分离自转移灶：胸腔积液 | 形态
Morphology | 上皮样，贴壁生长 | 培养基和添加剂
Complete Growth Medium and Culture Conditions | 人乳腺癌细胞MCF7培养液配方(100 ml)：
MEM (Invitrogen, 11090081)88 ml
FBS (Gibco)10 ml
Glutamax(invitrogen 35050061) 1 ml
Non-essential Amino Acids, 100X (Invitrogen, 11140050) 1 ml
Sodium Pyruvate 100 mM Solution (Invitrogen 11360070) 1 m
human recombinant insulin(SIGMA, 91077C-1G) 终浓度0.01 mg/ml
参考传代周期：5-7天
参考传代比例：1:3
参考换液频率：每周2-3次
冻存液配方：完全培养液95%，DMSO 5%
气相：空气，95%；二氧化碳，5%。温度：37摄氏度。
注意事项：
a) 该细胞贴壁较慢，建议复苏、传代后让细胞贴壁48-72h后再进行后续实验操作。
b) 使用0.25%(w/v) Trypsin-0.53 mM EDTA消化细胞。
c) MCF7是一种生长缓慢的细胞系，它会以松散的三维团簇的形式出现，并伴有一些漂浮的活细胞。悬浮的活细胞可以通过离心后重新加入到培养瓶中培养。在复苏后前二代该细胞会出现贴壁缓慢的情况，是正常现象。松散的三维团簇细胞慢慢会扩散形成一个扁平的单层细胞。如果生长速度比正常情况慢，可以尝试另一批FBS(即血清批次的促生长能力可能不同)。此外，将血清浓度增加到20%也可能有助于改善生长。 | 供应限制
Permits and Restrictions | A。仅供研究之用。 | | REFERENCE | Sugarman BJ , et al. Recombinant human tumor necrosis factor-alpha: effects on proliferation of normal and transformed cells in vitro. Science 230: 943-945, 1985. PubMed: 3933111 Takahashi K , Suzuki K . Association of insulin-like growth-factor-I-induced DNA synthesis with phosphorylation and nuclear exclusion of p53 in human breast cancer MCF-7 cells. Int. J. Cancer 55: 453-458, 1993. PubMed: 8375929 Brandes LJ , Hermonat MW . Receptor status and subsequent sensitivity of subclones of MCF-7 human breast cancer cells surviving exposure to diethylstilbestrol. Cancer Res. 43: 2831-2835, 1983. PubMed: 6850594 Lan MS , et al. Polypeptide core of a human pancreatic tumor mucin antigen. Cancer Res. 50: 2997-3001, 1990. PubMed: 2334903 Pratt SE , Pollak MN . Estrogen and antiestrogen modulation of MCF7 human breast cancer cell proliferation is associated with specific alterations in accumulation of insulin-like growth factor-binding proteins in conditioned media. Cancer Res. 53: 5193-5198, 1993. PubMed: 7693333 Huguet EL , et al. Differential expression of human Wnt genes 2, 3, 4, and 7B in human breast cell lines and normal and disease states of human breast tissue. Cancer Res. 54: 2615-2621, 1994. PubMed: 8168088 Soule HD , et al. A human cell line from a pleural effusion derived from a breast carcinoma. J. Natl. Cancer Inst. 51: 1409-1416, 1973. PubMed: 4357757 Bellet D , et al. Malignant transformation of nontrophoblastic cells is associated with the expression of chorionic gonadotropin beta genes normally transcribed in trophoblastic cells. Cancer Res. 57: 516-523, 1997. PubMed: 9012484 Littlewood-Evans AJ , et al. The osteoclast-associated protease cathepsin K is expressed in human breast carcinoma. Cancer Res. 57: 5386-5390, 1997. PubMed: 9393764 Komarova EA , et al. Intracellular localization of p53 tumor suppressor protein in gamma-irradiated cells is cell cycle regulated and determined by the nucleus. Cancer Res. 57: 5217-5220, 1997. PubMed: 9393737 van Dijk MA , et al. A functional assay in yeas for the human estrogen receptor displays wild-type and variant estrogen receptor messenger RNAs present in breast carcinoma. Cancer Res. 57: 3478-3485, 1997. PubMed: 9270016 Landers JE , et al. Translational enhancement of mdm2 oncogene expression in human tumor cells containing a stabilized wild-type p53 protein. Cancer Res. 57: 3562-3568, 1997. PubMed: 9270029 Umekita Y , et al. Human prostate tumor growth in athymic mice: inhibition by androgens and stimulation by finasteride. Proc. Natl. Acad. Sci. USA 93: 11802-11807, 1996. PubMed: 8876218 Zamora-Leon SP , et al. Expression of the fructose transporter GLUT5 in human breast cancer. Proc. Natl. Acad. Sci. USA 93: 1847-1852, 1996. PubMed: 8700847 Geiger T , et al. Antitumor activity of a PKC-alpha antisense oligonucleotide in combination with standard chemotherapeutic agents against various human tumors transplanted into nude mice. Anticancer Drug Des. 13: 35-45, 1998. PubMed: 9474241 Jang SI , et al. Activator protein 1 activity is involved in the regulation of the cell type-specific expression from the proximal promoter of the human profilaggrin gene. J. Biol. Chem. 271: 24105-24114, 1996. PubMed: 8798649 Lee JH , et al. The proximal promoter of the human transglutaminase 3 gene. J. Biol. Chem. 271: 4561-4568, 1996. PubMed: 8626812 Chang K , Pastan I . Molecular cloning of mesothelin, a differentiation antigen present on mesothelium, mesotheliomas, and ovarian cancers. Proc. Natl. Acad. Sci. USA 93: 136-140, 1996. PubMed: 8552591 Zhu X , et al. Cell cycle-dependent modulation of telomerase activity in tumor cells. Proc. Natl. Acad. Sci. USA 93: 6091-6095, 1996. PubMed: 8650224 Bacus SS , et al. Differentiation of cultured human breast cancer cells (AU-565 and MCF-7) associated with loss of cell surface HER-2/neu antigen. Mol. Carcinog. 3: 350-362, 1990. PubMed: 1980588 Acta Biochimica et Biophysica Sinica 34(2):165-170,2002 Chin J Oncol. 24(4):327-330,2002 Jiangsu Med J. 28(9):641-643,2002 Zhejiang Med 24(10):586-588,2002 Chin J Oncol. 25(4):332-334,2003 Chinese Journal of Cancer 22(3):257-261,2003 Chinese Journal of Cancer 22(4):380-384,2003 The Practical Journal of Cancer 18(5):457-459,2003 Research in Tumor Prev Treat 30(3):210-213,2003 China J Cancer Prev Treat 10(6):583-585,2003 J First Mil Med Univ 23(7):731-733,2003 Acta Academae Medicinae Miltaris Tertiae 25(2):151-154,2003 Acta Universitatis Medicinalis Nanjing (Natural Science) 23(2):134-137,2003 |
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