RW.4小鼠胚胎干细胞
产品介绍
目录号
Serial | SCSP-224 | 标识符
Identifier | CSTR:19375.09.3101MOUSCSP224 | 描述
Description | RW.4细胞对多能性标记物和碱性磷酸酶活性染色呈阳性，并且被证明具有生殖能力。每个批次均通过本库支原体检测，结果为阴性。 | 动物种别
Organism | 小鼠，129X1/SvJ品系 | 性别
Gender | 雄 | 组织来源
Tissue and Cell Type | 小鼠胚胎内细胞团 | 形态
Morphology | 球形克隆，贴壁生长 | 培养基和添加剂
Complete Growth Medium and Culture Conditions | mES完全培养液配方(600 ml)：
DMEM (Invitrogen 12430) 497 ml
ES级FBS (biochrom S4115) 90 ml
Glutamax (Invitrogen 35050) 6 ml
NEAA (Invitrogen 11140) 6 ml
LIF (Millipore ESG1107) 60 μl(终浓度为1000U/ml)
β-Mer (Invitrogen 21985) 1.5 ml
气相：空气，95%；二氧化碳，5%。温度：37摄氏度。 | 供应限制
Permits and Restrictions | A。仅供研究之用。 | | REFERENCE | Hug BA, et al. Analysis of mice containing a targeted deletion of beta-globin locus control region 5' hypersensitive site 3. Mol. Cell. Biol. 16: 2906-2912, 1996. PubMed: 8649401Matise MP, et al. Production of targeted Embryonic Stem Cell Clones. In AL Joyner (Ed.), Gene Targeting: A Practical Approach. Oxford University Press, Oxford, 101-132, 1999.Fabricius D, Bonde S, Zavazava N. Induction of stable mixed chimerism by embryonic stem cells requires functional Fas/FasL engagement. Transplantation 79(9): 1040-1044, 2005. PubMed:15880040Conte D, et al. Inhibitor of apoptosis protein cIAP2 is essential for lipopolysaccharide-induced macrophage survival. Mol. Cell Biol. 26(2): 699-708, 2006. PubMed: 16382159Westervelt P, et al. High-penetrance mouse model of acute promyelocytic leukemia with very low levels of PML-RARalpha expression. Blood 102(5): 1857-1865, 2003. PubMed: 12750176Schumacher A, et al. Staurosporine is a potent activator of neuronal, glial, and "CNS stem cell-like" neurosphere differentiation in murine embryonic stem cells. Mol. Cell Neurosci. 23: 669-680, 2003. PubMed: 12932446Lee MH, et al. Simvastatin suppresses self-renewal of mouse embryonic stem cells by inhibiting RhoA geranylgeranylation. Stem Cells 25: 1654-1663, 2007. PubMed: 17464088 |
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